BMRB Entry 11220
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11220
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Title: Solution structure of the second SH3 domain of human KIAA0769 protein
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the second SH3 domain of human KIAA0769 protein" . ., .-..
Assembly members:
SH3 domain, polymer, 73 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGVCFVKALYDYEGQ
TDDELSFPEGAIIRILNKEN
QDDDGFWEGEFNGRIGVFPS
VLVEELSSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 297 |
| 15N chemical shifts | 72 |
| 1H chemical shifts | 454 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | second SH3 domain of human KIAA0769 protein | 1 |
Entities:
Entity 1, second SH3 domain of human KIAA0769 protein 73 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | VAL | CYS | PHE | ||||
| 2 | VAL | LYS | ALA | LEU | TYR | ASP | TYR | GLU | GLY | GLN | ||||
| 3 | THR | ASP | ASP | GLU | LEU | SER | PHE | PRO | GLU | GLY | ||||
| 4 | ALA | ILE | ILE | ARG | ILE | LEU | ASN | LYS | GLU | ASN | ||||
| 5 | GLN | ASP | ASP | ASP | GLY | PHE | TRP | GLU | GLY | GLU | ||||
| 6 | PHE | ASN | GLY | ARG | ILE | GLY | VAL | PHE | PRO | SER | ||||
| 7 | VAL | LEU | VAL | GLU | GLU | LEU | SER | SER | GLY | PRO | ||||
| 8 | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.24 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
Delta v4.3, JEOL - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9296, Kobayashi N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- JEOL ECA 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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