BMRB Entry 11222
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11222
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Title: Solution structure of the first SH3 domain of human KIAA0769 protein
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Izumi, K.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Izumi, K.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first SH3 domain of human KIAA0769 protein" . ., .-..
Assembly members:
SH3 domain, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGTLRNYPLTCKVVY
SYKASQPDELTIEEHEVLEV
IEDGDMEDWVKARNKVGQVG
YVPEKYLQFPTSSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 328 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 508 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | first SH3 domain of human KIAA0769 protein | 1 |
Entities:
Entity 1, first SH3 domain of human KIAA0769 protein 78 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | THR | LEU | ARG | ||||
| 2 | ASN | TYR | PRO | LEU | THR | CYS | LYS | VAL | VAL | TYR | ||||
| 3 | SER | TYR | LYS | ALA | SER | GLN | PRO | ASP | GLU | LEU | ||||
| 4 | THR | ILE | GLU | GLU | HIS | GLU | VAL | LEU | GLU | VAL | ||||
| 5 | ILE | GLU | ASP | GLY | ASP | MET | GLU | ASP | TRP | VAL | ||||
| 6 | LYS | ALA | ARG | ASN | LYS | VAL | GLY | GLN | VAL | GLY | ||||
| 7 | TYR | VAL | PRO | GLU | LYS | TYR | LEU | GLN | PHE | PRO | ||||
| 8 | THR | SER | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
Delta v4.3, JEOL - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9296, Kobayashi N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- JEOL ECA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts