BMRB Entry 11236
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11236
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Title: Solution structure of the fourth Ig-like domain from myosin light chain kinase, smooth muscle
Deposition date: 2010-07-23 Original release date: 2011-08-03
Authors: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayahsi, F.; Yokoyama, S.
Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fourth Ig-like domain from myosin light chain kinase, smooth muscle" . ., .-..
Assembly members:
Ig domain, polymer, 99 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Ig domain: GSSGSSGMEVAPSFSSVLKD
CAVIEGQDFVLQCSVRGTPV
PRITWLLNGQPIQYARSTCE
AGVAELHIQDALPEDHGTYT
CLAENALGQVSCSAWVTVH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 400 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 637 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ig domain | 1 |
Entities:
Entity 1, Ig domain 99 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | GLU | VAL | ||||
| 2 | ALA | PRO | SER | PHE | SER | SER | VAL | LEU | LYS | ASP | ||||
| 3 | CYS | ALA | VAL | ILE | GLU | GLY | GLN | ASP | PHE | VAL | ||||
| 4 | LEU | GLN | CYS | SER | VAL | ARG | GLY | THR | PRO | VAL | ||||
| 5 | PRO | ARG | ILE | THR | TRP | LEU | LEU | ASN | GLY | GLN | ||||
| 6 | PRO | ILE | GLN | TYR | ALA | ARG | SER | THR | CYS | GLU | ||||
| 7 | ALA | GLY | VAL | ALA | GLU | LEU | HIS | ILE | GLN | ASP | ||||
| 8 | ALA | LEU | PRO | GLU | ASP | HIS | GLY | THR | TYR | THR | ||||
| 9 | CYS | LEU | ALA | GLU | ASN | ALA | LEU | GLY | GLN | VAL | ||||
| 10 | SER | CYS | SER | ALA | TRP | VAL | THR | VAL | HIS |
Samples:
sample_1: Ig domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
Delta v4.3, JEOL - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- JEOL ECA 700 MHz
Related Database Links:
| PDB |
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