BMRB Entry 11253
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11253
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Title: Solution Structure of the Ubiquitin-like Domain from Mouse Hypothetical 1700011N24Rik Protein
Deposition date: 2010-08-09 Original release date: 2011-08-17
Authors: Zhao, C.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Zhao, C.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of the Ubiquitin-like Domain from Mouse Hypothetical 1700011N24Rik Protein" . ., .-..
Assembly members:
Ubiquitin-like Domain, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Ubiquitin-like Domain: GSSGSSGMLITVYCVRRDLT
EVTFSLQVNPDFELSNFRVL
CELESGVPAEEAQIVYMEQL
LTDDHCSLGSYGLKDGDMVV
LLQKDNVGLRTPGRTPSGPS
SG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 385 |
15N chemical shifts | 95 |
1H chemical shifts | 643 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Ubiquitin-like Domain | 1 |
Entities:
Entity 1, Ubiquitin-like Domain 102 residues - Formula weight is not available
1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | LEU | ILE | ||||
2 | THR | VAL | TYR | CYS | VAL | ARG | ARG | ASP | LEU | THR | ||||
3 | GLU | VAL | THR | PHE | SER | LEU | GLN | VAL | ASN | PRO | ||||
4 | ASP | PHE | GLU | LEU | SER | ASN | PHE | ARG | VAL | LEU | ||||
5 | CYS | GLU | LEU | GLU | SER | GLY | VAL | PRO | ALA | GLU | ||||
6 | GLU | ALA | GLN | ILE | VAL | TYR | MET | GLU | GLN | LEU | ||||
7 | LEU | THR | ASP | ASP | HIS | CYS | SER | LEU | GLY | SER | ||||
8 | TYR | GLY | LEU | LYS | ASP | GLY | ASP | MET | VAL | VAL | ||||
9 | LEU | LEU | GLN | LYS | ASP | ASN | VAL | GLY | LEU | ARG | ||||
10 | THR | PRO | GLY | ARG | THR | PRO | SER | GLY | PRO | SER | ||||
11 | SER | GLY |
Samples:
sample_1: Ubiquitin-like Domain, [U-13C; U-15N], 1.17 mM; Phosphate Na 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298.0 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B., A. - data analysis
Kujira v0.860, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
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