BMRB Entry 11266
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11266
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Title: Solution Structure of the N-terminal Ubiquitin-like Domain in Human Np95/ICBP90-like Ring Finger Protein (NIRF)
Deposition date: 2010-08-09 Original release date: 2011-08-18
Authors: Zhao, C.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Zhao, C.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the N-terminal Ubiquitin-like Domain in Human Np95/ICBP90-like Ring Finger Protein (NIRF)" . ., .-..
Assembly members:
ubiquitin-like domain, polymer, 89 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
ubiquitin-like domain: GSSGSSGMWIQVRTIDGSKT
CTIEDVSRKATIEELRERVW
ALFDVRPECQRLFYRGKQLE
NGYTLFDYDVGLNDIIQLLV
RPDSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 368 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 581 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ubiquitin-like domain | 1 |
Entities:
Entity 1, ubiquitin-like domain 89 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | TRP | ILE | ||||
| 2 | GLN | VAL | ARG | THR | ILE | ASP | GLY | SER | LYS | THR | ||||
| 3 | CYS | THR | ILE | GLU | ASP | VAL | SER | ARG | LYS | ALA | ||||
| 4 | THR | ILE | GLU | GLU | LEU | ARG | GLU | ARG | VAL | TRP | ||||
| 5 | ALA | LEU | PHE | ASP | VAL | ARG | PRO | GLU | CYS | GLN | ||||
| 6 | ARG | LEU | PHE | TYR | ARG | GLY | LYS | GLN | LEU | GLU | ||||
| 7 | ASN | GLY | TYR | THR | LEU | PHE | ASP | TYR | ASP | VAL | ||||
| 8 | GLY | LEU | ASN | ASP | ILE | ILE | GLN | LEU | LEU | VAL | ||||
| 9 | ARG | PRO | ASP | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: ubiquitin-like domain, [U-13C; U-15N], 1.91 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.921, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB68317 BAC31223 BAC38081 BAC81739 BAE00280 |
| GB | AAH28397 AAH60241 AAI48950 AAM33798 AAM33799 |
| REF | NP_001095740 NP_659122 NP_690856 XP_001142916 XP_002742966 |
| SP | Q7TMI3 Q96PU4 |
| TPG | DAA26932 |
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