BMRB Entry 11328
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11328
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Title: Solution structure of the B-box domain of the zinc finger FYVE domain-containing protein 19 from Mus musculus
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Miyamoto, K.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the B-box domain of the zinc finger FYVE domain-containing protein 19 from Mus musculus" . ., .-..
Assembly members:
zf-B box, polymer, 67 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
zf-B box: GSSGSSGLPWCCICNEDATL
RCAGCDGDLYCARCFREGHD
NFDLKEHQTSPYHPRRPCQE
HSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 232 |
| 15N chemical shifts | 53 |
| 1H chemical shifts | 347 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zf-B box | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, zf-B box 67 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LEU | PRO | TRP | ||||
| 2 | CYS | CYS | ILE | CYS | ASN | GLU | ASP | ALA | THR | LEU | ||||
| 3 | ARG | CYS | ALA | GLY | CYS | ASP | GLY | ASP | LEU | TYR | ||||
| 4 | CYS | ALA | ARG | CYS | PHE | ARG | GLU | GLY | HIS | ASP | ||||
| 5 | ASN | PHE | ASP | LEU | LYS | GLU | HIS | GLN | THR | SER | ||||
| 6 | PRO | TYR | HIS | PRO | ARG | ARG | PRO | CYS | GLN | GLU | ||||
| 7 | HIS | SER | GLY | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: zf-B_box, [U-13C; U-15N], 1.08 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9321, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAB23993 |
| GB | AAH18420 EDL27942 EDL27943 |
| REF | NP_082330 XP_005603199 XP_006500266 XP_006500267 XP_006500268 |
| SP | Q9DAZ9 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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