BMRB Entry 11344

Name: Solution structure of the RING domain of the human RING finger protein 141
Published: 2011-08-19

Click here to enlarge.

PDB ID: 2ecn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11344
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Solution structure of the RING domain of the human RING finger protein 141

Deposition date: 2010-08-10 Original release date: 2011-08-19

Authors: Miyamoto, K.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Miyamoto, K.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the RING domain of the human RING finger protein 141" . ., .-..

Assembly members:
RING domain, polymer, 70 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free

Entity Sequences (FASTA):
RING domain: GSSGSSGRVKQLTDEEECCI CMDGRADLILPCAHSFCQKC IDKWSDRHRNCPICRLQMTG ANESSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	255
15N chemical shifts	61
1H chemical shifts	401

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING domain 70 residues - Formula weight is not available

1	GLY	SER	SER	GLY	SER	SER	GLY	ARG	VAL	LYS
2	GLN	LEU	THR	ASP	GLU	GLU	GLU	CYS	CYS	ILE
3	CYS	MET	ASP	GLY	ARG	ALA	ASP	LEU	ILE	LEU
4	PRO	CYS	ALA	HIS	SER	PHE	CYS	GLN	LYS	CYS
5	ILE	ASP	LYS	TRP	SER	ASP	ARG	HIS	ARG	ASN
6	CYS	PRO	ILE	CYS	ARG	LEU	GLN	MET	THR	GLY
7	ALA	ASN	GLU	SER	SER	GLY	PRO	SER	SER	GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.31 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	2ECN
DBJ	BAB24742 BAB27871 BAB29874 BAE32570 BAF82453
EMBL	CAH92252
GB	AAF30180 AAH18104 AAH18553 AAH88675 AAI09814
REF	NP_001001800 NP_001035656 NP_001041484 NP_001088870 NP_001126318
SP	Q2XNS1 Q32L15 Q5R7K8 Q6IV57 Q8WVD5
TPG	DAA22251

Biological Magnetic Resonance Data Bank