BMRB Entry 11380
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11380
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Title: Solution structure of the 4th zinc finger domain of Zinc finger protein 278
Deposition date: 2010-09-08 Original release date: 2011-09-07
Authors: Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Citation: Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the 4th zinc finger domain of Zinc finger protein 278" . ., .-..
Assembly members:
zinc finger domain, UNP residues 408-448, polymer, 54 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
zinc finger domain, UNP residues 408-448: GSSGSSGSVGKPYICQSCGK
GFSRPDHLNGHIKQVHTSER
PHKCQVWVSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 190 |
| 15N chemical shifts | 44 |
| 1H chemical shifts | 287 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zinc finger domain, UNP residues 408-448 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, zinc finger domain, UNP residues 408-448 54 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | VAL | GLY | ||||
| 2 | LYS | PRO | TYR | ILE | CYS | GLN | SER | CYS | GLY | LYS | ||||
| 3 | GLY | PHE | SER | ARG | PRO | ASP | HIS | LEU | ASN | GLY | ||||
| 4 | HIS | ILE | LYS | GLN | VAL | HIS | THR | SER | GLU | ARG | ||||
| 5 | PRO | HIS | LYS | CYS | GLN | VAL | TRP | VAL | SER | GLY | ||||
| 6 | PRO | SER | SER | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: zinc finger domain, UNP residues 408-448, [U-13C; U-15N], 1.08 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20060702, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.1, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| GB | AAG09036 AAG09037 KFQ43058 |
| REF | XP_004934428 XP_004934429 XP_004934430 XP_004934431 XP_004934432 |
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