BMRB Entry 15023
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15023
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Title: 1H, and 15N Chemical Shift Assignments for the N-terminal domain of Myxococcus xantus CarA protein. PubMed: 17233828
Deposition date: 2006-11-13 Original release date: 2007-02-08
Authors: Jimenez, M. Angeles; Padmanabhan, S.; Gonzalez, Carlos; Perez-Marin, Mari; Elias-Arnanz, Montserrat; Murillo, Francisco; Rico, Manuel
Citation: Navarro-Aviles, G.; Jimenez, M. Angeles; Perez-Marin, Mari; Gonzalez, Carlos; Rico, Manuel; Murillo, Francisco; Elias-Arnanz, Montserrat; Padmanabhan, S.. "Structural basis for operator and anti-repressor recognition by Myxococcus xanthus CarA repressor" Mol. Microbiol. 63, 980-994 (2007).
Assembly members:
CarA(Nter), polymer, 81 residues, Formula weight is not available
Natural source: Common Name: Myxococcus xanthus Taxonomy ID: 34 Superkingdom: Bacteria Kingdom: not available Genus/species: Myxococcus xanthus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CarA(Nter): GSHMTLRIRTIARMTGIREA
TLRAWERRYGFPRPLRSEGN
NYRVYSREEVEAVRRVARLI
QEEGLSVSEAIAQVKTEPPR
E
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 561 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CarA(Nter | 1 |
Entities:
Entity 1, CarA(Nter 81 residues - Formula weight is not available
Residues -2 to 0 represent an N-terminal cloning tag.
| 1 | GLY | SER | HIS | MET | THR | LEU | ARG | ILE | ARG | THR | ||||
| 2 | ILE | ALA | ARG | MET | THR | GLY | ILE | ARG | GLU | ALA | ||||
| 3 | THR | LEU | ARG | ALA | TRP | GLU | ARG | ARG | TYR | GLY | ||||
| 4 | PHE | PRO | ARG | PRO | LEU | ARG | SER | GLU | GLY | ASN | ||||
| 5 | ASN | TYR | ARG | VAL | TYR | SER | ARG | GLU | GLU | VAL | ||||
| 6 | GLU | ALA | VAL | ARG | ARG | VAL | ALA | ARG | LEU | ILE | ||||
| 7 | GLN | GLU | GLU | GLY | LEU | SER | VAL | SER | GLU | ALA | ||||
| 8 | ILE | ALA | GLN | VAL | LYS | THR | GLU | PRO | PRO | ARG | ||||
| 9 | GLU |
Samples:
sample_1: CarA(Nter)0.5 – 1.0 mM; H2O 90%; D2O 10%; DSS0.1 – 0.5 mM; sodium chloride 100 mM; sodium phosphate 50 mM
sample_2: CarA(Nter)0.5 – 1.0 mM; D2O 100%; DSS0.1 – 0.5 mM; sodium chloride 100 mM; sodium phosphate 50 mM
sample_3: CarA(Nter), [U-100% 15N], 0.5 – 1.0 mM; H2O 90%; D2O 10%; DSS0.1 – 0.5 mM; sodium chloride 100 mM; sodium phosphate 50 mM
sample_conditions_1: ionic strength: 0.150 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_3 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_2 | isotropic | sample_conditions_1 |
Software:
SPARKY, T Goddard - chemical shift assignment
NMR spectrometers:
- Bruker AV 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts