BMRB Entry 15107
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15107
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Title: NMR structure of D4P/K7G mutant of GPM12 PubMed: 18473359
Deposition date: 2007-01-22 Original release date: 2008-06-25
Authors: Terada, T.; Satoh, D.; Mikawa, T.; Ito, Y.; Shimizu, K.
Citation: Terada, Tohru; Satoh, Daisuke; Mikawa, Tsutomu; Ito, Yutaka; Shimizu, Kentaro. "Understanding the roles of amino acid residues in tertiary structure formation of chignolin by using molecular dynamics simulation" Proteins 73, 621-631 (2008).
Assembly members:
GPM12, polymer, 10 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
GPM12: GYDPATGTFG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 53 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GPM12 | 1 |
Entities:
Entity 1, GPM12 10 residues - Formula weight is not available
| 1 | GLY | TYR | ASP | PRO | ALA | THR | GLY | THR | PHE | GLY |
Samples:
sample: GPM12 2 mM; sodium phosphate 20 mM; D2O, [U-2H], 10%; H2O 90%
sample_conditions_1: ionic strength: 20 mM; pH: 5.5; pressure: 1 atm; temperature: 277 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample | isotropic | sample_conditions_1 |
| 2D TOCSY | sample | isotropic | sample_conditions_1 |
| 2D ROESY | sample | isotropic | sample_conditions_1 |
Software:
xwinnmr v3.5, Bruker BioSpin Corporation - collection
AZARA v2.7, Wayne Boucher - processing
ANSIG v3.3 for OpenGL version 1.0.6, Per Kraulis, Takeshi Nishimura - data analysis
CNS v1.1, Axel T.Brunger et al. - refinement, structure solution
NMR spectrometers:
- Bruker DRX 600 MHz