BMRB Entry 15323
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15323
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Title: Solution structure of Manduca sexta moricin PubMed: 18265434
Deposition date: 2007-06-21 Original release date: 2008-02-29
Authors: Gong, Yuxi; Dai, Huaien; Rayaprolu, Subrahmanyam; Huang, Rudan; Prakash, Om; Jiang, Haobo
Citation: Dai, Huaien; Rayaprolu, Subrahmanyam; Gong, Yuxi; Huang, Rudan; Prakash, Om; Jiang, Haobo. "Solution structure, antibacterial activity, and expression profile of Manduca sexta moricin" J. Pept. Sci. 14, 855-863 (2008).
Assembly members:
moricin, polymer, 42 residues, Formula weight is not available
Natural source: Common Name: Manduca sexta Taxonomy ID: 7130 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Manduca sexta
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
moricin: GKIPVKAIKQAGKVIGKGLR
AINIAGTTHDVVSFFRPKKK
KH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 263 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | moricin | 1 |
Entities:
Entity 1, moricin 42 residues - Formula weight is not available
| 1 | GLY | LYS | ILE | PRO | VAL | LYS | ALA | ILE | LYS | GLN | ||||
| 2 | ALA | GLY | LYS | VAL | ILE | GLY | LYS | GLY | LEU | ARG | ||||
| 3 | ALA | ILE | ASN | ILE | ALA | GLY | THR | THR | HIS | ASP | ||||
| 4 | VAL | VAL | SER | PHE | PHE | ARG | PRO | LYS | LYS | LYS | ||||
| 5 | LYS | HIS |
Samples:
sample_1: moricin 2 mM; CD3OH 100%
sample_conditions_1: ionic strength: 20 mM; pH: 5.7; pressure: 1 atm; temperature: 283.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
SPARKY v3.111, Goddard - data analysis
NMR spectrometers:
- Varian UnityPlus 500 MHz