BMRB Entry 15325
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15325
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Title: C-terminal domain of ORF1p from mouse LINE-1 PubMed: 17569664
Deposition date: 2007-06-21 Original release date: 2007-07-24
Authors: Januszyk, Kurt; Clubb, Robert
Citation: Januszyk, Kurt; Li, Patrick; Villareal, Valerie; Branciforte, Dan; Wu, Haihong; Xie, Yongming; Feigon, Juli; Loo, Joseph; Martin, Sandra; Clubb, Robert. "Identification and solution structure of a highly conserved C-terminal domain within ORF1p required for retrotransposition of long interspersed nuclear element-1" J. Biol. Chem. 282, 24893-24904 (2007).
Assembly members:
CTD, polymer, 88 residues, 7649.985 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CTD: MQVTYKGRPIRITPDFSPET
MKARRAWTDVIQTLREHKCQ
PRLLYPAKLSITIDGETKVF
HDKTKFTQYLSTNPALQRII
TEKKQYKD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 413 |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 630 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CTD | 1 |
Entities:
Entity 1, CTD 88 residues - 7649.985 Da.
| 1 | MET | GLN | VAL | THR | TYR | LYS | GLY | ARG | PRO | ILE | ||||
| 2 | ARG | ILE | THR | PRO | ASP | PHE | SER | PRO | GLU | THR | ||||
| 3 | MET | LYS | ALA | ARG | ARG | ALA | TRP | THR | ASP | VAL | ||||
| 4 | ILE | GLN | THR | LEU | ARG | GLU | HIS | LYS | CYS | GLN | ||||
| 5 | PRO | ARG | LEU | LEU | TYR | PRO | ALA | LYS | LEU | SER | ||||
| 6 | ILE | THR | ILE | ASP | GLY | GLU | THR | LYS | VAL | PHE | ||||
| 7 | HIS | ASP | LYS | THR | LYS | PHE | THR | GLN | TYR | LEU | ||||
| 8 | SER | THR | ASN | PRO | ALA | LEU | GLN | ARG | ILE | ILE | ||||
| 9 | THR | GLU | LYS | LYS | GLN | TYR | LYS | ASP |
Samples:
sample_1: entity, [U-99% 13C; U-99% 15N], 1 mM; Phosphate buffer 50 mM; NaCl 200 mM; DTT 5 mM; D2O 7%
sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| EMBL | CAA27362 CAI02523 CRH42983 CRH55784 CRH56877 |
| GB | AAA39397 AAA66023 AAA67726 AAL17969 AAL17971 |
| REF | WP_057184893 WP_057247702 WP_057256795 XP_676702 XP_727753 |
| SP | P11260 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
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or all simulated shifts