BMRB Entry 16103

Name: 1H, 13C, and 15N chemical shift assignments for murine FAIM-CTD
Published: 2009-04-22

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PDB ID: 2kd2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16103
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 13C, and 15N chemical shift assignments for murine FAIM-CTD PubMed: 19168072

Deposition date: 2009-01-01 Original release date: 2009-04-22

Authors: Hemond, Michael; Wagner, Gerhard

Citation: Hemond, Michael; Rothstein, Thomas; Wagner, Gerhard. "Fas Apoptosis Inhibitory Molecule contains a novel beta sandwich in contact with a partially ordered domain" J. Mol. Biol. 386, 1024-1037 (2009).

Assembly members:
FAIM-CTD, polymer, 94 residues, Formula weight is not available

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
FAIM-CTD: GPLGSENRSKTTSTWVLRLD GEDLRVVLEKDTMDVWCNGQ KMETAGEFVDDGTETHFSVG NHDCYIKAVSSGKRKEGIIH TLIVDNREIPELTQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
RDCs
- HN_RDC

Data type	Count
13C chemical shifts	364
15N chemical shifts	96
1H chemical shifts	561
residual dipolar couplings	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FAIM-CTD monomer	1

Entities:

Entity 1, FAIM-CTD monomer 94 residues - Formula weight is not available

Residues 1-5 are derived from a non-native protease site and polypeptide linker

1

GLY

PRO

LEU

GLY

SER

GLU

ASN

ARG

SER

LYS

2

THR

SER

THR

TRP

VAL

LEU

ARG

LEU

ASP

3

GLY

GLU

ASP

LEU

ARG

VAL

LEU

GLU

LYS

4

ASP

THR

MET

ASP

VAL

TRP

CYS

ASN

GLY

GLN

5

LYS

MET

GLU

THR

ALA

GLY

GLU

PHE

VAL

ASP

6

ASP

GLY

THR

GLU

THR

HIS

PHE

SER

VAL

GLY

7

ASN

HIS

ASP

CYS

TYR

ILE

LYS

ALA

VAL

SER

8

SER

GLY

LYS

ARG

LYS

GLU

GLY

ILE

HIS

9

THR

LEU

ILE

VAL

ASP

ASN

ARG

GLU

ILE

PRO

10

GLU

LEU

THR

GLN

Samples:

sample_1: FAIM-CTD, [U-99% 13C; U-99% 15N], 0.25 – 0.5 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_2: FAIM-CTD, [U-99% 13C; U-99% 15N], 0.25 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM; Pf1 filamentous phage 0.6% w/v

sample_3: FAIM-CTD, [U-99% 13C], 1.8 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_4: FAIM-CTD, [U-10% 13C], 0.5 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_5: FAIM-CTD, [U-99% 15N], 0.25 – 0.9 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_6: FAIM-CTD 1.4 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.3; pressure: 1.0 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	anisotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_5	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_5	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_6	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D CCH-COSY	sample_3	isotropic	sample_conditions_1

Software:

NMRPipe v2.4 Rev 2006.095.11.35, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Varian INOVA 500 MHz
Bruker Avance 900 MHz

PDB	2KD2
DBJ	BAB24225
GB	AAD23879 AAH79662 EDL20994 EDL20995
REF	NP_001116323 NP_035940
SP	Q9WUD8

Biological Magnetic Resonance Data Bank