BMRB Entry 16111
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16111
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Title: Sequence-specific 1H, 15N and 13C resonance assignments of Art v 1: a proline-rich allergen of Artemisia vulgaris pollen PubMed: 19636957
Deposition date: 2009-01-08 Original release date: 2009-03-17
Authors: Razzera, Guilherme; Gadermaier, Gabriele; Almeida, Marcius; Ferreira, Fatima; Almeida, Fabio; Valente, Ana Paula
Citation: Razzera, Guilherme; Gadermaier, Gabriele; Almeida, Marcius; Ferreira, Fatima; Almeida, Fabio; Valente, Ana Paula. "Sequence-specific 1H, 15N and 13C resonance assignments of Art v 1: a proline-rich allergen of Artemisia vulgaris pollen" Biomol. NMR Assignments 3, 103-106 (2009).
Assembly members:
Art_v_1, polymer, 108 residues, 10800.0 Da.
Natural source: Common Name: common wormwood Taxonomy ID: 4220 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Artemisia vulgaris
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Art_v_1: AGSKLCEKTSKTYSGKCDNK
KCDKKCIEWEKAQHGACHKR
EAGKESCFCYFDCSKSPPGA
TPAPPGAAPPPAAGGSPSPP
ADGGSPPPPADGGSPPVDGG
SPPPPSTH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 293 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 603 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Art v 1 | 1 |
Entities:
Entity 1, Art v 1 108 residues - 10800.0 Da.
Proline-rich allergen of Artemisia vulgaris pollen
| 1 | ALA | GLY | SER | LYS | LEU | CYS | GLU | LYS | THR | SER | ||||
| 2 | LYS | THR | TYR | SER | GLY | LYS | CYS | ASP | ASN | LYS | ||||
| 3 | LYS | CYS | ASP | LYS | LYS | CYS | ILE | GLU | TRP | GLU | ||||
| 4 | LYS | ALA | GLN | HIS | GLY | ALA | CYS | HIS | LYS | ARG | ||||
| 5 | GLU | ALA | GLY | LYS | GLU | SER | CYS | PHE | CYS | TYR | ||||
| 6 | PHE | ASP | CYS | SER | LYS | SER | PRO | PRO | GLY | ALA | ||||
| 7 | THR | PRO | ALA | PRO | PRO | GLY | ALA | ALA | PRO | PRO | ||||
| 8 | PRO | ALA | ALA | GLY | GLY | SER | PRO | SER | PRO | PRO | ||||
| 9 | ALA | ASP | GLY | GLY | SER | PRO | PRO | PRO | PRO | ALA | ||||
| 10 | ASP | GLY | GLY | SER | PRO | PRO | VAL | ASP | GLY | GLY | ||||
| 11 | SER | PRO | PRO | PRO | PRO | SER | THR | HIS |
Samples:
sample_1: Art v 1, [U-99% 13C; U-99% 15N], 1 mM; Potassium Acetate 10 mM
sample_conditions_1: ionic strength: 0.01 M; pH: 5.3; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| HCAN | sample_1 | isotropic | sample_conditions_1 |
| HCACON | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection, data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA v1.84, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts