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BMRB Entry 16404

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16404
MolProbity Validation Chart

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Title: Solution structure of human ubiquitin conjugating enzyme Ube2g2   PubMed: 20014027

Deposition date: 2009-07-10 Original release date: 2009-12-18

Authors: Ju, Tingting; Bocik, William; Majumdar, Ananya; Tolman, Joel

Citation: Ju, Tingting; Bocik, William; Majumdar, Ananya; Tolman, Joel. "Solution structure and dynamics of human ubiquitin conjugating enzyme Ube2g2."  Proteins 78, 1291-1301 (2010).

Assembly members:
Human ubiquitin-conjugating enzyme E2G2 isoform 1, polymer, 165 residues, 18582.391 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Human ubiquitin-conjugating enzyme E2G2 isoform 1: MAGTALKRLMAEYKQLTLNP PEGIVAGPMNEENFFEWEAL IMGPEDTCFEFGVFPAILSF PLDYPLSPPKMRFTCEMFHP NIYPDGRVCISILHAPGDDP MGYESSAERWSPVQSVEKIL LSVVSMLAEPNDESGANVDA SKMWRDDREQFYKIAKQIVQ KSLGL

Data sets:
Data typeCount
13C chemical shifts715
15N chemical shifts166
1H chemical shifts1086

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human ubiquitin-conjugating enzyme E2G2 isoform 11

Entities:

Entity 1, Human ubiquitin-conjugating enzyme E2G2 isoform 1 165 residues - 18582.391 Da.

1   METALAGLYTHRALALEULYSARGLEUMET
2   ALAGLUTYRLYSGLNLEUTHRLEUASNPRO
3   PROGLUGLYILEVALALAGLYPROMETASN
4   GLUGLUASNPHEPHEGLUTRPGLUALALEU
5   ILEMETGLYPROGLUASPTHRCYSPHEGLU
6   PHEGLYVALPHEPROALAILELEUSERPHE
7   PROLEUASPTYRPROLEUSERPROPROLYS
8   METARGPHETHRCYSGLUMETPHEHISPRO
9   ASNILETYRPROASPGLYARGVALCYSILE
10   SERILELEUHISALAPROGLYASPASPPRO
11   METGLYTYRGLUSERSERALAGLUARGTRP
12   SERPROVALGLNSERVALGLULYSILELEU
13   LEUSERVALVALSERMETLEUALAGLUPRO
14   ASNASPGLUSERGLYALAASNVALASPALA
15   SERLYSMETTRPARGASPASPARGGLUGLN
16   PHETYRLYSILEALALYSGLNILEVALGLN
17   LYSSERLEUGLYLEU

Samples:

sample_1: Human ubiquitin-conjugating enzyme E2G2 isoform 1, [U-99% 13C; U-99% 15N], 1.0 mM; phosphate buffer 20 mM; NaCl 50 mM; EDTA 0.5 mM; TCEP 1 mM; H2O 90%; D2O 10%

sample_2: Human ubiquitin-conjugating enzyme E2G2 isoform 1, [U-99% 15N], 0.7 mM; phosphate buffer 20 mM; NaCl 50 mM; EDTA 0.5 mM; TCEP 1 mM; H2O 90%; D2O 10%; bacteriophage Pf1 8 mg/ml

sample_3: Human ubiquitin-conjugating enzyme E2G2 isoform 1, [U-99% 13C; U-99% 15N], 1.0 mM; phosphate buffer 20 mM; NaCl 50 mM; EDTA 0.5 mM; TCEP 1 mM; H2O 90%; D2O 10%; polyacrylamide gel 4.5%

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
2D_NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
IPAP-HSQCsample_2anisotropicsample_conditions_1
IPAP-HSQCsample_3anisotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Varian Inova 800 MHz
  • Bruker Avance II 600 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAE22948 BAE27256 BAE32604 BAF83318 BAG53675
EMBL CAB90551 CAD38846 CAH89573
GB AAF21503 AAH01738 AAH08351 AAH10321 AAH11569
REF NP_001069796 NP_001099850 NP_001124691 NP_001181099 NP_001189418
SP P60604 P60605 Q17QG5 Q5RF84
TPG DAA32954

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