BMRB Entry 17152
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PDB ID:
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Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17152
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Title: Helical hairpin structure of a novel antimicrobial peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli) PubMed: 21561864
Deposition date: 2010-08-27 Original release date: 2011-05-04
Authors: Nolde, Svetlana; Barinov, Nikolay; Balashova, Tamara; Arseniev, Alexander
Citation: Nolde, Svetlana; Vassilevski, Alexander; Rogozhin, Eugene; Barinov, Nikolay; Balashova, Tamara; Samsonova, Olga; Baranov, Yuri; Feofanov, Alexey; Egorov, Tsezi; Arseniev, Alexander; Grishin, Eugene. "Disulfide-stabilized helical hairpin structure and activity of a novel antifungal peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)." J. Biol. Chem. 286, 25145-25153 (2011).
Assembly members:
EcAMP1, polymer, 37 residues, 4288.794 Da.
Natural source: Common Name: Barnyard grass Taxonomy ID: 90397 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Echinochloa crus-galli
Experimental source: Production method: purified from the natural source Host organism: Echinochloa crusgalli
Entity Sequences (FASTA):
EcAMP1: GSGRGSCRSQCMRRHEDEPW
RVQECVSQCRRRRGGGD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 227 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EcAMP1 | 1 |
Entities:
Entity 1, EcAMP1 37 residues - 4288.794 Da.
| 1 | GLY | SER | GLY | ARG | GLY | SER | CYS | ARG | SER | GLN | ||||
| 2 | CYS | MET | ARG | ARG | HIS | GLU | ASP | GLU | PRO | TRP | ||||
| 3 | ARG | VAL | GLN | GLU | CYS | VAL | SER | GLN | CYS | ARG | ||||
| 4 | ARG | ARG | ARG | GLY | GLY | GLY | ASP |
Samples:
sample_1: EcAMP1 0.7 mg/ml; KOH 10 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 10 mM; pH: 6.62; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 800 MHz