BMRB Entry 17194
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17194
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Title: Oxki4a, spider derived antimicrobial peptide PubMed: 21933345
Deposition date: 2010-09-14 Original release date: 2012-08-01
Authors: Vassilevski, Alexander; Dubovskii, Peter; Samsonova, Olga; Egorova, Natalya; Kozlov, Sergey; Feofanov, Alexei; Arseniev, Alexander; Grishin, Eugene
Citation: Dubovskii, Peter; Vassilevski, Alexander; Samsonova, Olga; Egorova, Natalya; Kozlov, Sergey; Feofanov, Alexei; Arseniev, Alexander; Grishin, Eugene. "Novel lynx spider toxin shares common molecular architecture with defense peptides from frog skin" FEBS J. 278, 4382-4393 (2011).
Assembly members:
entity, polymer, 30 residues, 3624.556 Da.
Natural source: Common Name: lynx spider Taxonomy ID: 666126 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oxyopes takobius
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: GIRCPKSWKCKAFKQRVLKR
LLAMLRQHAF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 233 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Oxki4a | 1 |
Entities:
Entity 1, Oxki4a 30 residues - 3624.556 Da.
| 1 | GLY | ILE | ARG | CYS | PRO | LYS | SER | TRP | LYS | CYS | |
| 2 | LYS | ALA | PHE | LYS | GLN | ARG | VAL | LEU | LYS | ARG | |
| 3 | LEU | LEU | ALA | MET | LEU | ARG | GLN | HIS | ALA | PHE |
Samples:
sample_1: Oxki4a 1 ± 0.1 mM; dodecylphosphocholine-d38 60 ± 1 mM; D2O 5%; H2O 95%
sample-2: Oxki4a 1 mM; dodecylphosphocholine-d38 60 mM; D2O 100%
sample_conditions_1: ionic strength: 0 M; pH: 4.0; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample-2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample-2 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample-2 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels C., Xia T., Billeter M., Guntert P., Wuthrich K. - chemical shift assignment
xwinnmr, Bruker Biospin - collection
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz