BMRB Entry 17771
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17771
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Title: Resonance Assignments of Calmodulin Complexed with the Calmodulin-Binding Domain of Olfactory Nucleotide Gated Ion Channel PubMed: 22877078
Deposition date: 2011-07-08 Original release date: 2012-09-20
Authors: Deli, Irene; Chyan, Chia Lin
Citation: Irene, Deli; Huang, Jian-Wen; Chung, Tse-Yu; Li, Feng-Yin; Tzen, Jason T-C; Lin, Ta-Hsien; Chyan, Chia-Lin. "Binding orientation and specificity of calmodulin to rat olfactory cyclic nucleotide-gated ion channel." J. Biomol. Struct. Dyn. 31, 414-425 (2013).
Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
OLFp, polymer, 28 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
OLFp: TPRRGRGGFQRIVRLVGVIR
DWANKNFR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 1371 |
| 15N chemical shifts | 348 |
| 1H chemical shifts | 2278 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Chain 1 | 1 |
| 2 | Chain 2 | 2 |
| 3 | Calcium ion | 3 |
Entities:
Entity 1, Chain 1 148 residues - Formula weight is not available
| 1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
| 4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
| 6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
| 7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
| 8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
| 9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
| 10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
| 12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
| 13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
| 14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
| 15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, Chain 2 28 residues - Formula weight is not available
| 1 | THR | PRO | ARG | ARG | GLY | ARG | GLY | GLY | PHE | GLN | ||||
| 2 | ARG | ILE | VAL | ARG | LEU | VAL | GLY | VAL | ILE | ARG | ||||
| 3 | ASP | TRP | ALA | ASN | LYS | ASN | PHE | ARG |
Entity 3, Calcium ion - Ca - 40.078 Da.
| 1 | CA |
Samples:
CaM-OLFp: Calmodulin, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; OLFp, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; H2O 90%; D2O 10%
sample_conditions: pH: 6.7; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | CaM-OLFp | isotropic | sample_conditions |
| 2D 1H-13C HSQC | CaM-OLFp | isotropic | sample_conditions |
| 2D 1H-1H TOCSY | CaM-OLFp | isotropic | sample_conditions |
| 2D DQF-COSY | CaM-OLFp | isotropic | sample_conditions |
| 2D 1H-1H NOESY | CaM-OLFp | isotropic | sample_conditions |
| 3D HNCA | CaM-OLFp | isotropic | sample_conditions |
| 3D HN(CO)CA | CaM-OLFp | isotropic | sample_conditions |
| 3D HNCO | CaM-OLFp | isotropic | sample_conditions |
| 3D HN(CA)CO | CaM-OLFp | isotropic | sample_conditions |
| 3D HNCACB | CaM-OLFp | isotropic | sample_conditions |
| 3D C(CO)NH | CaM-OLFp | isotropic | sample_conditions |
| 3D HBHA(CO)NH | CaM-OLFp | isotropic | sample_conditions |
| 3D H(CCO)NH | CaM-OLFp | isotropic | sample_conditions |
| 3D HCCH-TOCSY | CaM-OLFp | isotropic | sample_conditions |
| 3D HCCH-COSY | CaM-OLFp | isotropic | sample_conditions |
| 3D 1H-15N-edited TOCSY HSQC | CaM-OLFp | isotropic | sample_conditions |
| 3D simultaneous 1H-13C/15N-edited NOESY-HSQC | CaM-OLFp | isotropic | sample_conditions |
Software:
AURELIA, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310 |
| PDB | |
| DBJ | BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 |
| EMBL | CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA39135 CAQ34767 |
| GB | AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAD41473 |
| PIR | JC1305 MCON |
| PRF | 0409298A 0608335A 1614345A |
| REF | NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_037060 XP_008756353 |
| SP | O02367 O16305 O96081 P02594 P05932 Q00195 |
| TPG | DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts