BMRB Entry 17889
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17889
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Title: The polyserine tract of Nasonia vitripennis Vg residues 351-385 PubMed: 22573762
Deposition date: 2011-08-29 Original release date: 2012-05-22
Authors: Havukainen, Heli; Halskau, Oyvind
Citation: Havukainen, Heli; Underhaug, Jarl; Wolschin, Florian; Amdam, Gro; Halskau, Oyvind. "A vitellogenin polyserine cleavage site: highly disordered conformation protected from proteolysis by phosphorylation." J. Exp. Biol. 215, 1837-1846 (2012).
Assembly members:
NvVg, polymer, 35 residues, 4046.233 Da.
Natural source: Common Name: Jewel Wasp Taxonomy ID: 7425 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Nasonia vitripennis
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
NvVg: EHKHSDESTSESFESIADNN
DDSYFQRKPKLTEAP
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 37 |
| 1H chemical shifts | 222 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NvVg | 1 |
Entities:
Entity 1, NvVg 35 residues - 4046.233 Da.
| 1 | GLU | HIS | LYS | HIS | SER | ASP | GLU | SER | THR | SER | ||||
| 2 | GLU | SER | PHE | GLU | SER | ILE | ALA | ASP | ASN | ASN | ||||
| 3 | ASP | ASP | SER | TYR | PHE | GLN | ARG | LYS | PRO | LYS | ||||
| 4 | LEU | THR | GLU | ALA | PRO |
Samples:
sample_1: NvVg, [U-15N]-ILE, 20 w/v; sodium phosphate 50 mM; sodium azide 0.02%; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.06 M; pH: 6.7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v1.2, Linge, O, . - structure solution
NMR spectrometers:
- Bruker AV600 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts