BMRB Entry 17912
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17912
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: The third SH3 domain of R85FL PubMed: 23892081
Deposition date: 2011-09-02 Original release date: 2012-09-14
Authors: Jiang, Ya-Jun; Hu, Hongyu
Citation: Jiang, Ya-Jun; Zhou, Chen-Jie; Zhou, Zi-Ren; Wu, Meng; Hu, Hong-Yu. "Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7." FEBS Lett. 587, 2905-2911 (2013).
Assembly members:
third SH3 domain of R85FL, polymer, 65 residues, 7581.400 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
third SH3 domain of R85FL: QTSQDLFSYQALYSYIPQND
DELELRDGDIVDVMEKCDDG
WFVGTSRRTKQFGTFPGNYV
KPLYL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 229 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 396 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | third SH3 domain of R85FL | 1 |
Entities:
Entity 1, third SH3 domain of R85FL 65 residues - 7581.400 Da.
| 1 | GLN | THR | SER | GLN | ASP | LEU | PHE | SER | TYR | GLN | ||||
| 2 | ALA | LEU | TYR | SER | TYR | ILE | PRO | GLN | ASN | ASP | ||||
| 3 | ASP | GLU | LEU | GLU | LEU | ARG | ASP | GLY | ASP | ILE | ||||
| 4 | VAL | ASP | VAL | MET | GLU | LYS | CYS | ASP | ASP | GLY | ||||
| 5 | TRP | PHE | VAL | GLY | THR | SER | ARG | ARG | THR | LYS | ||||
| 6 | GLN | PHE | GLY | THR | PHE | PRO | GLY | ASN | TYR | VAL | ||||
| 7 | LYS | PRO | LEU | TYR | LEU |
Samples:
sample_1: sodium phosphate 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.12 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA, Linge, O, . - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 17913 |
| PDB | |
| DBJ | BAA92534 BAG58006 BAG62137 BAG65026 BAG72789 |
| EMBL | CAB55947 CAD34588 CAE45892 CAH89601 CAH90184 |
| GB | AAD27647 AAF22175 AAI52464 AAI60134 AAK37563 |
| REF | NP_001030126 NP_001030127 NP_001030128 NP_001030129 NP_001068637 |
| SP | Q9BX66 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts