BMRB Entry 18009
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18009
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Title: NMR structure of the lambda 5 22-45 peptide PubMed: 23124203
Deposition date: 2011-10-19 Original release date: 2012-10-22
Authors: Elantak, Latifa; Espeli, Marion; Boned, Annie; Bornet, Olivier; Breton, Caroline; Feracci, Mikael; Roche, Philippe; Guerlesquin, Francoise; Schiff, Claudine
Citation: Elantak, Latifa; Espeli, Marion; Boned, Annie; Bornet, Olivier; Bonzi, Jeremy; Gauthier, Laurent; Feracci, Mikael; Roche, Philippe; Guerlesquin, Francoise; Schiff, Claudine. "Structural basis for galectin-1-dependent pre-B cell receptor (pre-BCR) activation." J. Biol. Chem. 287, 44703-44713 (2012).
Assembly members:
entity, polymer, 24 residues, 2856.299 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: SRSSLRSRWGRFLLQRGSWT
GPRC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 159 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | lambda_5_22-45_peptide | 1 |
Entities:
Entity 1, lambda_5_22-45_peptide 24 residues - 2856.299 Da.
| 1 | SER | ARG | SER | SER | LEU | ARG | SER | ARG | TRP | GLY | ||||
| 2 | ARG | PHE | LEU | LEU | GLN | ARG | GLY | SER | TRP | THR | ||||
| 3 | GLY | PRO | ARG | CYS |
Samples:
sample_1: potassium phosphate 20 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.2; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAI46548 |
| GB | AAA36096 AAA36100 AAH12293 ABM84504 ABM85566 |
| REF | NP_064455 XP_003282108 XP_003814324 XP_004063189 XP_004063194 |
| SP | P15814 |