BMRB Entry 18014
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18014
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Title: A Myristoylated Polyproline Type II Helix Functions as a Novel Fusion Peptide During Cell-Cell Membrane Fusion Induced by the Baboon Reovirus p15 FAST Protein
Deposition date: 2011-10-21 Original release date: 2011-11-29
Authors: Top, Deniz; Read, Jolene; Dawe, Sandra; Syvitski, Raymond; Duncan, Roy
Citation: Top, Deniz; Read, Jolene; Dawe, Sandra; Syvitski, Raymond; Duncan, Roy. "A Myristoylated Polyproline Type II Helix Functions as a Novel Fusion Peptide During Cell-Cell Membrane Fusion Induced by the Baboon Reovirus p15 FAST Protein" J. Biol. Chem. ., .-..
Assembly members:
entity, polymer, 20 residues, 2156.466 Da.
Natural source: Common Name: Orthoreovirus Taxonomy ID: 10882 Superkingdom: Viruses Kingdom: not available Genus/species: Orthoreovirus not available
Experimental source: Production method: recombinant technology Host organism: Desulfovibrio desulfuricans
Entity Sequences (FASTA):
entity: GQRHSIVQPPAPPPNAFVEI
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Myristoylated Polyproline Type II Helix | 1 |
Entities:
Entity 1, Myristoylated Polyproline Type II Helix 20 residues - 2156.466 Da.
| 1 | GLY | GLN | ARG | HIS | SER | ILE | VAL | GLN | PRO | PRO | |
| 2 | ALA | PRO | PRO | PRO | ASN | ALA | PHE | VAL | GLU | ILE |
Samples:
sample_1: entity 5 ± 1 mM
sample_conditions_1: ionic strength: 0 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.1, Goddard - chemical shift assignment, data analysis
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization, refinement
NMR spectrometers:
- Bruker DRX 500 MHz