BMRB Entry 18042

Name: Structure of EDC3:DCP2
Published: 2012-01-23

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PDB ID: 4a54
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18042
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structure of EDC3:DCP2 PubMed: 22085934

Deposition date: 2011-11-07 Original release date: 2012-01-23

Authors: Truffault, Vincent

Citation: Fromm, Simon; Truffault, Vincent; Kamenz, Julia; Braun, Joerg; Hoffmann, Niklas; Izaurralde, Elisa; Sprangers, Remco. "The structural basis of Edc3- and Scd6-mediated activation of the Dcp1:Dcp2 mRNA decapping complex." EMBO J. 31, 279-290 (2011).

Assembly members:
EDC3, polymer, 96 residues, Formula weight is not available
DCP2, polymer, 48 residues, Formula weight is not available

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
EDC3: MGMSVADFYGSNVEVLLNND SKARGVITNFDSSNSILQLR LANDSTKSIVTKDIKDLRIL PKNEIMPKNGTKSPSTNSTK LKSAETYSSKNKWSMD
DCP2: GATTKEKNISVDVDADASSQ LLSLLKSSTAPSDLATPQPS TFPQPPVE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	436
15N chemical shifts	101
1H chemical shifts	678

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EDC3	1
2	DCP2	2

Entities:

Entity 1, EDC3 96 residues - Formula weight is not available

1

MET

GLY

MET

SER

VAL

ALA

ASP

PHE

TYR

GLY

2

SER

ASN

VAL

GLU

VAL

LEU

ASN

ASP

3

SER

LYS

ALA

ARG

GLY

VAL

ILE

THR

ASN

PHE

4

ASP

SER

ASN

SER

ILE

LEU

GLN

LEU

ARG

5

LEU

ALA

ASN

ASP

SER

THR

LYS

SER

ILE

VAL

6

THR

LYS

ASP

ILE

LYS

ASP

LEU

ARG

ILE

LEU

7

PRO

LYS

ASN

GLU

ILE

MET

PRO

LYS

ASN

GLY

8

THR

LYS

SER

PRO

SER

THR

ASN

SER

THR

LYS

9

LEU

LYS

SER

ALA

GLU

THR

TYR

SER

LYS

10

ASN

LYS

TRP

SER

MET

ASP

Entity 2, DCP2 48 residues - Formula weight is not available

1

GLY

ALA

THR

LYS

GLU

LYS

ASN

ILE

SER

2

VAL

ASP

VAL

ASP

ALA

ASP

ALA

SER

GLN

3

LEU

SER

LEU

LYS

SER

THR

ALA

4

PRO

SER

ASP

LEU

ALA

THR

PRO

GLN

PRO

SER

5

THR

PHE

PRO

GLN

PRO

VAL

GLU

Samples:

sample_1: EDC3, [U-100% 15N], 0.7 mM; DCP2, [U-100% 15N], 0.7 mM; NaPO4 50 mM; imidazole 10 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 800 MHz

BMRB	18041
PDB	4A53 4A54 4A54
EMBL	CAA22671 CAB11648
REF	NP_595858 NP_593780
SP	O94752 O13828

Biological Magnetic Resonance Data Bank