BMRB Entry 18111
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18111
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Title: Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain complex
Deposition date: 2011-11-29 Original release date: 2012-01-25
Authors: Liu, Minhao
Citation: Liu, Minhao; Matthews, Stephen; James, Ellen; Sheppard, Carol. "Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain complex" Mol. Cell ., .-..
Assembly members:
entity_1, polymer, 84 residues, 6635.446 Da.
entity_2, polymer, 84 residues, 6795.774 Da.
Natural source: Common Name: not available Taxonomy ID: 10760 Superkingdom: not available Kingdom: not available Genus/species: Bacteriophage T7
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH
MSNVNTGSLSVDNKKFWATV
ESSEHSFEVPIYAETLDEAL
ELAEWQYVPAGFEVTRVRPC
VAPK
entity_2: MGSSHHHHHHSSGLVPRGSH
MKEPAILAEISGIVSFGKET
KGKRRLVITPVDGSDPYEEM
IPKWRQLNVFEGERVERGDV
ISDG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 499 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 762 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 84 residues - 6635.446 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | SER | ASN | VAL | ASN | THR | GLY | SER | LEU | SER | ||||
| 4 | VAL | ASP | ASN | LYS | LYS | PHE | TRP | ALA | THR | VAL | ||||
| 5 | GLU | SER | SER | GLU | HIS | SER | PHE | GLU | VAL | PRO | ||||
| 6 | ILE | TYR | ALA | GLU | THR | LEU | ASP | GLU | ALA | LEU | ||||
| 7 | GLU | LEU | ALA | GLU | TRP | GLN | TYR | VAL | PRO | ALA | ||||
| 8 | GLY | PHE | GLU | VAL | THR | ARG | VAL | ARG | PRO | CYS | ||||
| 9 | VAL | ALA | PRO | LYS |
Entity 2, entity_2 84 residues - 6795.774 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | LYS | GLU | PRO | ALA | ILE | LEU | ALA | GLU | ILE | ||||
| 4 | SER | GLY | ILE | VAL | SER | PHE | GLY | LYS | GLU | THR | ||||
| 5 | LYS | GLY | LYS | ARG | ARG | LEU | VAL | ILE | THR | PRO | ||||
| 6 | VAL | ASP | GLY | SER | ASP | PRO | TYR | GLU | GLU | MET | ||||
| 7 | ILE | PRO | LYS | TRP | ARG | GLN | LEU | ASN | VAL | PHE | ||||
| 8 | GLU | GLY | GLU | ARG | VAL | GLU | ARG | GLY | ASP | VAL | ||||
| 9 | ILE | SER | ASP | GLY |
Samples:
sample_1: Gp2-Jaw complex, [U-100% 13C; U-100% 15N], 300 mM; Gp2-Jaw complex, [U-100% 13C; U-100% 15N], 300 mM; H2O 90%; D2O 10%; NaCl 50 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA, Linge, O'Donoghue and Nilges - structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DPX 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts