BMRB Entry 18175
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18175
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Title: 40-residue D23N beta amyloid fibril PubMed: 22403062
Deposition date: 2012-01-03 Original release date: 2012-02-06
Authors: Qiang, Wei; Yau, Wai-Ming; Luo, Yongquan; Mattson, Mark; Tycko, Robert
Citation: Qiang, Wei; Yau, Wai-Ming; Luo, Yongquan; Mattson, Mark; Tycko, Robert. "Antiparallel -sheet architecture in Iowa-mutant -amyloid fibrils." Proc. Natl. Acad. Sci. U.S.A. 109, 4443-4448 (2012).
Assembly members:
entity, polymer, 26 residues, 2650.167 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: QKLVFFAENVGSNKGAIIGL
MVGGVV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 74 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | D23N beta amyloid fibril, chain 1 | 1 |
| 2 | D23N beta amyloid fibril, chain 2 | 1 |
| 3 | D23N beta amyloid fibril, chain 3 | 1 |
| 4 | D23N beta amyloid fibril, chain 4 | 1 |
| 5 | D23N beta amyloid fibril, chain 5 | 1 |
| 6 | D23N beta amyloid fibril, chain 6 | 1 |
| 7 | D23N beta amyloid fibril, chain 7 | 1 |
| 8 | D23N beta amyloid fibril, chain 8 | 1 |
Entities:
Entity 1, D23N beta amyloid fibril, chain 1 26 residues - 2650.167 Da.
| 1 | GLN | LYS | LEU | VAL | PHE | PHE | ALA | GLU | ASN | VAL | ||||
| 2 | GLY | SER | ASN | LYS | GLY | ALA | ILE | ILE | GLY | LEU | ||||
| 3 | MET | VAL | GLY | GLY | VAL | VAL |
Samples:
sample_1: entity, [U-13C], 25 uM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-13C fpRFDR | sample_1 | solid | sample_conditions_1 |
| 2D 13C-13C RAD | sample_1 | solid | sample_conditions_1 |
| 2D 13C-13C CHHC | sample_1 | solid | sample_conditions_1 |
| 1D 13C PITHIRDs-CT | sample_1 | solid | sample_conditions_1 |
Software:
X-PLOR NIH v2.28.1, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Varian InfinityPlus 400 MHz