BMRB Entry 18400
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18400
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Title: RNA structural dynamics are modulated through anti-folding by chaperones
Deposition date: 2012-04-15 Original release date: 2012-05-31
Authors: Fuertig, Boris; Doetsch, Martina; Stampfl, Sabine; Kontaxis, Georg; Schroeder, Renee
Citation: Fuertig, Boris; Doetsch, Martina; Stampfl, Sabine; Kontaxis, Georg; Schroeder, Renee. "Chaperones modulate RNA structural dynamics through anti-folding" Not known ., .-..
Assembly members:
CTD-StpA, polymer, 45 residues, 5039.755 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CTD-StpA: RQPRPAKYKFTDVNGETKTW
TGQGRTPKPIAQALAEGKSL
DDFLI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 182 |
| 15N chemical shifts | 43 |
| 1H chemical shifts | 309 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CTD-StpA | 1 |
Entities:
Entity 1, CTD-StpA 45 residues - 5039.755 Da.
| 1 | ARG | GLN | PRO | ARG | PRO | ALA | LYS | TYR | LYS | PHE | ||||
| 2 | THR | ASP | VAL | ASN | GLY | GLU | THR | LYS | THR | TRP | ||||
| 3 | THR | GLY | GLN | GLY | ARG | THR | PRO | LYS | PRO | ILE | ||||
| 4 | ALA | GLN | ALA | LEU | ALA | GLU | GLY | LYS | SER | LEU | ||||
| 5 | ASP | ASP | PHE | LEU | ILE |
Samples:
sample_1: CTD-StpA, [U-99% 13C; U-99% 15N], 1 mM
sample_conditions_1: ionic strength: 0.075 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAA16535 BAB36953 BAG78446 BAI26931 BAI31961 |
| EMBL | CAA49146 CAP77108 CAQ33006 CAQ87964 CAQ99591 |
| GB | AAA64940 AAC75716 AAG57777 AAN44190 AAN81670 |
| REF | NP_311557 NP_417155 NP_708483 WP_000055597 WP_000115370 |
| SP | P0ACG1 P0ACG2 P0ACG3 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts