BMRB Entry 18440
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18440
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Title: Analog of the fragment 197-221 of 1- adrenoreceptor
Deposition date: 2012-05-04 Original release date: 2013-05-03
Authors: Bushuev, Valery; Zykov, K.; Bespalova, Z.; Bibilashvili, R.; Efremov, E.; Golitsyn, S.; Kaznacheeva, L.; Kuzhetsova, T.; Levashov, P.; Masenko, V.; Mironova, N.; Molokoedov, F.; Rutkevich, P.; Rvacheva, A.; Sharf, T.; Sidorova, M.; Vlasik, T.; Yanushevskaya, E.
Citation: Bushuev, Valery. "Not applicable" To be Published ., .-..
Assembly members:
entity, polymer, 25 residues, 2941.249 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis Host organism: none
Entity Sequences (FASTA):
entity: NCWRAESDEARRCYNDPKCS
DSVCK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 63 |
| 1H chemical shifts | 147 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Analog of the fragment 197-221 of 1- adrenoreceptor | 1 |
Entities:
Entity 1, Analog of the fragment 197-221 of 1- adrenoreceptor 25 residues - 2941.249 Da.
| 1 | ASN | CYS | TRP | ARG | ALA | GLU | SER | ASP | GLU | ALA | ||||
| 2 | ARG | ARG | CYS | TYR | ASN | ASP | PRO | LYS | CYS | SER | ||||
| 3 | ASP | SER | VAL | CYS | LYS |
Samples:
sample_1: entity 2 mM; sodium azide 1 mM; sodium acetate, [U-100% 2H], 20 mM; H2O 90%; D2O 10%
sample_2: entity 2 mM; sodium azide 1 mM; sodium acetate, [U-100% 2H], 20 mM; D2O 100%
sample_conditions_1: ionic strength: 21 mM; pH: 5.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_2 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr v1.3, Bruker Biospin - collection, processing
CARA v1.9.5, Keller and Wuthrich - chemical shift assignment, peak picking
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker DPX 500 MHz
Related Database Links:
| PDB |