BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18493

Title: High resolution structure of DsbB C41S by joint calculation with solid-state NMR and X-ray data   PubMed: 23416557

Deposition date: 2012-05-30 Original release date: 2013-02-27

Authors: Tang, Ming; Sperling, Lindsay; Schwieters, Charles; Nesbitt, Anna; Gennis, Robert; Rienstra, Chad

Citation: Tang, Ming; Nesbitt, Anna; Sperling, Lindsay; Berthold, Deborah; Schwieters, Charles; Gennis, Robert; Rienstra, Chad. "Structure of the disulfide bond generating membrane protein DsbB in the lipid bilayer."  J. Mol. Biol. 425, 1670-1682 (2013).

Assembly members:
DsbB, polymer, 176 residues, 16663.053 Da.
entity_2, polymer, 239 residues, 24077.834 Da.
entity_3, polymer, 221 residues, 23024.885 Da.
entity_UQ1, non-polymer, 250.290 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DsbB: MLRFLNQASQGRGAWLLMAF TALALELTALWFQHVMLLKP SVLCIYERVALFGVLGAALI GAIAPKTPLRYVAMVIWLYS AFRGVQLTYEHTMLQLYPSP FATCDFMVRFPEWLPLDKWV PQVFVASGDCAERQWDFLGL EMPQWLLGIFIAYLIVAVLV VISQPFKAKKRDLFGR
entity_2: MDSQAQVLILLLLWVSGTCG DIVMSQSPSSLAVSAGEKVT MSCKSSQSLLNSRTRKNYLA WYQQKPGQSPKLLIYWASTR ESGVPDRFTGSGSGTDFTLT ISSVQAEDLAVYYCKQSYNL YTFGGGTKLEIKRADAAPTV SIFPPSSEQLTSGGASVVCF LNNFYPKDINVKWKIDGSER QNGVLNSWTDQDSKDSTYSM SSTLTLTKDEYERHNSYTCE ATHKTSTSPIVKSFNRNEC
entity_3: EVQLVESGGGLVKPGGSLKL SCAASGFAFSSYDMSWVRQT PEKRLEWVAYISSGGGSTYY PDTVKGRFTISRDNAKNTLY LQMSSLKSEDTAMYYCARPD YRSYAMDYWGQGTSVTVSSA KTTAPSVYPLAPVCGDTTGS SVTLGCLVKGYFPEPVTLTW NSGSLSSGVHTFPAVLQSDL YTLSSSVTVTSSTWPSQSIT CNVAHPASSTKVDKKIEPRG P

Data sets:
Data typeCount
13C chemical shifts552
15N chemical shifts125
1H chemical shifts67

Additional metadata:

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Related Database Links:

PDB 2ZUQ 2HI7 2K73 2K74 2LEG 2LTQ 2ZUP 3E9J 1Q9K 1Q9L 1Q9O 1Q9W 2I9L 2R1W 2R1X 2R1Y 2R23 2R2B 2R2E 2R2H 3BPC 3HZK 3HZM 3HZV 3HZY 3I02 3IJH 3IJS 3IJY 3IKC 3KJ4 3SY0 3T4Y 3T65 3T77 4HGW
BMRB 15546 15966 18395
DBJ BAA36032 BAB35103 BAG76757 BAI24997 BAI30121
EMBL CAP75720 CAQ31687 CAQ98064 CAR02574 CAR12682 CAA75918
GB AAA23711 AAB25233 AAC74269 AAG56036 AAN42789 AAA53292 AAB47613 AAL04472 AEB66102 EDK98900 AAN06761
PIR H85696 G33932 PS0023
REF NP_309707 NP_415703 NP_707082 WP_000652474 WP_000943442
SP A1AAA8 P0A6M2 P0A6M3 P59343 Q0T5L6

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts