BMRB Entry 18548
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18548
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Title: 1H, 13C, and 15N resonance assignments of the monomeric human Fam96a PubMed: 23793605
Deposition date: 2012-06-25 Original release date: 2013-09-12
Authors: Xia, Bin; Ouyang, Bingjie
Citation: Ouyang, Bingjie; Wang, Lei; Wan, Shuo; Luo, Yang; Wang, Lu; Lin, Jian; Xia, Bin. "Solution structure of monomeric human FAM96A" J. Biomol. NMR 56, 387-392 (2013).
Assembly members:
Fam96a, polymer, 143 residues, Formula weight is not available
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Fam96a: MRQPRIMEEKALEVYDLIRT
IRDPEKPNTLEELEVVSESC
VEVQEINEEEYLVIIRFTPT
VPHCSLATLIGLCLRVKLQR
CLPFKHKLEIYISEGTHSTE
EDINKQINDKERVAAAMENP
NLREIVEQCVLEPDQLEHHH
HHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 595 |
| 15N chemical shifts | 136 |
| 1H chemical shifts | 1004 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fam96a | 1 |
Entities:
Entity 1, Fam96a 143 residues - Formula weight is not available
| 1 | MET | ARG | GLN | PRO | ARG | ILE | MET | GLU | GLU | LYS | ||||
| 2 | ALA | LEU | GLU | VAL | TYR | ASP | LEU | ILE | ARG | THR | ||||
| 3 | ILE | ARG | ASP | PRO | GLU | LYS | PRO | ASN | THR | LEU | ||||
| 4 | GLU | GLU | LEU | GLU | VAL | VAL | SER | GLU | SER | CYS | ||||
| 5 | VAL | GLU | VAL | GLN | GLU | ILE | ASN | GLU | GLU | GLU | ||||
| 6 | TYR | LEU | VAL | ILE | ILE | ARG | PHE | THR | PRO | THR | ||||
| 7 | VAL | PRO | HIS | CYS | SER | LEU | ALA | THR | LEU | ILE | ||||
| 8 | GLY | LEU | CYS | LEU | ARG | VAL | LYS | LEU | GLN | ARG | ||||
| 9 | CYS | LEU | PRO | PHE | LYS | HIS | LYS | LEU | GLU | ILE | ||||
| 10 | TYR | ILE | SER | GLU | GLY | THR | HIS | SER | THR | GLU | ||||
| 11 | GLU | ASP | ILE | ASN | LYS | GLN | ILE | ASN | ASP | LYS | ||||
| 12 | GLU | ARG | VAL | ALA | ALA | ALA | MET | GLU | ASN | PRO | ||||
| 13 | ASN | LEU | ARG | GLU | ILE | VAL | GLU | GLN | CYS | VAL | ||||
| 14 | LEU | GLU | PRO | ASP | GLN | LEU | GLU | HIS | HIS | HIS | ||||
| 15 | HIS | HIS | HIS |
Samples:
sample_1: Fam96a, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; EDTA 1 mM; DTT 50 mM; ARG 50 mM; GLU 50 mM; D2O 5%; H2O 95%
sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 18137 |
| PDB | |
| DBJ | BAB15496 BAB22285 BAC36966 BAE41803 BAG35105 |
| GB | AAH05745 AAH08865 AAI02257 AAI51429 ABQ82139 |
| REF | NP_001030282 NP_001092912 NP_080911 NP_115607 XP_002718208 |
| SP | Q3T0U7 Q9DCL2 Q9H5X1 |
| TPG | DAA25322 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts