BMRB Entry 18764
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18764
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Title: Phf19 links methylated lysine 36 of histone H3 to regulation of Polycomb activity PubMed: 23104054
Deposition date: 2012-10-04 Original release date: 2012-10-29
Authors: Lapinaite, Audrone; Simon, B.; Carlomagno, T.
Citation: Ballare, Cecilia; Lange, Martin; Lapinaite, Audrone; Martin, Gloria Mas; Morey, Lluis; Pascual, Gloria; Liefke, Robert; Simon, Bernd; Shi, Yang; Gozani, Or; Carlomagno, Teresa; Benitah, Salvador Aznar; Di Croce, Luciano. "Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity." Nat. Struct. Mol. Biol. 19, 1257-1265 (2012).
Assembly members:
PHD_FINGER_PROTEIN_19, polymer, 58 residues, 6714.6566 Da.
HISTONE_H3, polymer, 11 residues, 1258.5055 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI BL21
Entity Sequences (FASTA):
PHD_FINGER_PROTEIN_19: SKLTEGQYVLCRWTDGLYYL
GKIKRVSSSKQSCLVTFEDN
SKYWVLWKDIQHAGVPGE
HISTONE_H3: ATGGVXKPHRY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 198 |
| 15N chemical shifts | 59 |
| 1H chemical shifts | 447 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PHD FINGER PROTEIN 19 | 1 |
| 2 | HISTONE H3 | 2 |
Entities:
Entity 1, PHD FINGER PROTEIN 19 58 residues - 6714.6566 Da.
| 1 | SER | LYS | LEU | THR | GLU | GLY | GLN | TYR | VAL | LEU | ||||
| 2 | CYS | ARG | TRP | THR | ASP | GLY | LEU | TYR | TYR | LEU | ||||
| 3 | GLY | LYS | ILE | LYS | ARG | VAL | SER | SER | SER | LYS | ||||
| 4 | GLN | SER | CYS | LEU | VAL | THR | PHE | GLU | ASP | ASN | ||||
| 5 | SER | LYS | TYR | TRP | VAL | LEU | TRP | LYS | ASP | ILE | ||||
| 6 | GLN | HIS | ALA | GLY | VAL | PRO | GLY | GLU |
Entity 2, HISTONE H3 11 residues - 1258.5055 Da.
| 1 | ALA | THR | GLY | GLY | VAL | M3L | LYS | PRO | HIS | ARG | ||||
| 2 | TYR |
Samples:
sample_1: PHD FINGER PROTEIN 19, [U-13C; U-15N], 0.1 – 0.6 mM; HISTONE H3, [U-13C; U-15N], 0.1 – 0.6 mM
sample_conditions_1: ionic strength: 200.000 mM; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TRIPLE RESONANCE | sample_1 | solution | sample_conditions_1 |
| NOESY | sample_1 | solution | sample_conditions_1 |
| EDITED-FILTERED | sample_1 | solution | sample_conditions_1 |
| EDITED -FILTERED | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - collection
CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment
NMRView vany, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| UNP | PHF19_HUMAN H31_HUMAN |
| PDB | |
| DBJ | BAG53700 BAI45704 |
| EMBL | CAE45832 |
| GB | AAH22374 AAI08664 AAI25077 AAI25078 ADQ32204 |
| REF | NP_001009936 NP_001179644 NP_001273769 NP_001273772 NP_056466 |
| SP | Q5T6S3 |
| TPG | DAA26446 |
Download simulated HSQC data in one of the following formats:
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