BMRB Entry 18953
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18953
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Title: Solution Structure of gammaM7-Crystallin PubMed: 23597261
Deposition date: 2013-01-16 Original release date: 2013-06-04
Authors: Mahler, Bryon; Wu, Zhengrong
Citation: Mahler, Bryon; Chen, Yingwei; Ford, Jason; Thiel, Caleb; Wistow, Graeme; Wu, Zhengrong. "Structure and Dynamics of the Fish Eye Lens Protein, M7-Crystallin." Biochemistry 52, 3579-3587 (2013).
Assembly members:
entity, polymer, 174 residues, 20937.965 Da.
Natural source: Common Name: Zebrafish Taxonomy ID: 7955 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Danio rerio
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MGKIIFYEDRNFGGRYHECM
SDCADLHSYFNRCHSIRVES
GCFMVYDRTNFMGRQYFLRR
GEYPDYMRTMGMNDCVRSCR
MIPLHHGSFKMRLYEHSDMG
GRMMELMDDCPNLMDRFNMS
DFHSCHVMDGHWLVYEQPNY
TGRQFYLRPGEYRSYNDWGG
VTSRMGSIRRITDL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 648 |
| 15N chemical shifts | 165 |
| 1H chemical shifts | 817 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gammaM-crystallin | 1 |
Entities:
Entity 1, gammaM-crystallin 174 residues - 20937.965 Da.
| 1 | MET | GLY | LYS | ILE | ILE | PHE | TYR | GLU | ASP | ARG | ||||
| 2 | ASN | PHE | GLY | GLY | ARG | TYR | HIS | GLU | CYS | MET | ||||
| 3 | SER | ASP | CYS | ALA | ASP | LEU | HIS | SER | TYR | PHE | ||||
| 4 | ASN | ARG | CYS | HIS | SER | ILE | ARG | VAL | GLU | SER | ||||
| 5 | GLY | CYS | PHE | MET | VAL | TYR | ASP | ARG | THR | ASN | ||||
| 6 | PHE | MET | GLY | ARG | GLN | TYR | PHE | LEU | ARG | ARG | ||||
| 7 | GLY | GLU | TYR | PRO | ASP | TYR | MET | ARG | THR | MET | ||||
| 8 | GLY | MET | ASN | ASP | CYS | VAL | ARG | SER | CYS | ARG | ||||
| 9 | MET | ILE | PRO | LEU | HIS | HIS | GLY | SER | PHE | LYS | ||||
| 10 | MET | ARG | LEU | TYR | GLU | HIS | SER | ASP | MET | GLY | ||||
| 11 | GLY | ARG | MET | MET | GLU | LEU | MET | ASP | ASP | CYS | ||||
| 12 | PRO | ASN | LEU | MET | ASP | ARG | PHE | ASN | MET | SER | ||||
| 13 | ASP | PHE | HIS | SER | CYS | HIS | VAL | MET | ASP | GLY | ||||
| 14 | HIS | TRP | LEU | VAL | TYR | GLU | GLN | PRO | ASN | TYR | ||||
| 15 | THR | GLY | ARG | GLN | PHE | TYR | LEU | ARG | PRO | GLY | ||||
| 16 | GLU | TYR | ARG | SER | TYR | ASN | ASP | TRP | GLY | GLY | ||||
| 17 | VAL | THR | SER | ARG | MET | GLY | SER | ILE | ARG | ARG | ||||
| 18 | ILE | THR | ASP | LEU |
Samples:
sample_1: imidazole, [U-2H], 25 mM; potassium chloride 20 mM; DTT 2 mM; sodium azide 0.04%; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 20 mM; pH: 6.2; pressure: 1 atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-edited NOESY-HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 13C-edited HMQC-NOESY-HMQC | sample_1 | isotropic | sample_conditions_1 |
| RDC by HSQC-TROSY | sample_1 | anisotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
AQUA, Rullmann, Doreleijers and Kaptein - refinement
ProcheckNMR, Laskowski and MacArthur - refinement
TALOS, Cornilescu, Delaglio and Bax - data analysis
xwinnmr, Bruker Biospin - collection
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts