BMRB Entry 19059
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19059
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: ns5a308
Deposition date: 2013-02-27 Original release date: 2014-03-03
Authors: Montserret, Roland; Badillo, Aurelie; Hanoulle, Xavier; Lippens, Guy; Penin, Francois
Citation: Montserret, Roland; Badillo, Aurelie; Hanoulle, Xavier; Lippens, Guy; Penin, Francois. "ns5a308" J. Biol. Chem. ., .-..
Assembly members:
entity, polymer, 20 residues, 2414.867 Da.
Natural source: Common Name: not available Taxonomy ID: 333284 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C Virus
Experimental source: Production method: chemical synthesis Host organism: none
Entity Sequences (FASTA):
entity: KFPRAMPIWARPDYNPPLLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 81 |
| 15N chemical shifts | 14 |
| 1H chemical shifts | 149 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ns5a308 | 1 |
Entities:
Entity 1, ns5a308 20 residues - 2414.867 Da.
| 1 | LYS | PHE | PRO | ARG | ALA | MET | PRO | ILE | TRP | ALA | |
| 2 | ARG | PRO | ASP | TYR | ASN | PRO | PRO | LEU | LEU | GLU |
Samples:
sample_1: entity 10 ± 1 mM; sodium phosphate 20 ± 1 mM; sodium chloride 30 ± 1 mM; H20 95 ± 1 %; D20 5 ± 1 %
sample_2: entity, [U-100% 13C; U-100% 15N], 1 ± 0.2 mM; sodium phosphate 50 ± 1 mM; sodium chloride 50 ± 1 mM; H20 95 ± 1 %; D20 5 ± 1 %
sample_conditions_1: ionic strength: 40 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCOCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection
SPARKY, Goddard - chemical shift assignment, data analysis
X-PLOR NIH v2.30, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD73970 BAF56924 BAF56926 BAF56930 BAF56934 |
| EMBL | CAB41950 CAB41951 CAB46677 CAB46911 CAB46913 |
| GB | AAC41018 AAG22682 AAG22683 AAG22685 AAG22686 |
| SP | Q9WMX2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts