BMRB Entry 19146
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19146
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Title: Solution structure of RING domain of E3 ubiquitin ligase Doa10
Deposition date: 2013-04-06 Original release date: 2014-05-05
Authors: Lim, Jongsoo; Son, Woo-Sung
Citation: Ahn, Hee-Chul; Lim, Jongsoo; Son, Woo-Sung. "Solution structure of RING domain of E3 ubiquitin ligase Doa10" Not known ., .-..
Assembly members:
entity_1, polymer, 83 residues, 9220.431 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: VANEETDTATFNDDAPSGAT
CRICRGEATEDNPLFHPCKC
RGSIKYMHESCLLEWVASKN
IDISKPGADVKCDICHYPIQ
FKT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 349 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 542 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RING domain of E3 ubiquitin ligase Doa10 | 1 |
| 2 | ZINC ION_1 | 2 |
Entities:
Entity 1, RING domain of E3 ubiquitin ligase Doa10 83 residues - 9220.431 Da.
| 1 | VAL | ALA | ASN | GLU | GLU | THR | ASP | THR | ALA | THR | ||||
| 2 | PHE | ASN | ASP | ASP | ALA | PRO | SER | GLY | ALA | THR | ||||
| 3 | CYS | ARG | ILE | CYS | ARG | GLY | GLU | ALA | THR | GLU | ||||
| 4 | ASP | ASN | PRO | LEU | PHE | HIS | PRO | CYS | LYS | CYS | ||||
| 5 | ARG | GLY | SER | ILE | LYS | TYR | MET | HIS | GLU | SER | ||||
| 6 | CYS | LEU | LEU | GLU | TRP | VAL | ALA | SER | LYS | ASN | ||||
| 7 | ILE | ASP | ILE | SER | LYS | PRO | GLY | ALA | ASP | VAL | ||||
| 8 | LYS | CYS | ASP | ILE | CYS | HIS | TYR | PRO | ILE | GLN | ||||
| 9 | PHE | LYS | THR |
Entity 2, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: MES, [U-100% 13C; U-100% 15N], 0.5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Varian INOVA 900 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | GAA24084 |
| EMBL | CAA54133 CAA86961 |
| GB | AHY75969 AJP39437 AJR36730 AJR36925 AJR37116 |
| REF | NP_012234 |
| SP | P40318 |
| TPG | DAA08517 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts