BMRB Entry 19368
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19368
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Title: Structure of Pex14 in complex with Pex5 LVxEF motif PubMed: 24235149
Deposition date: 2013-07-16 Original release date: 2013-11-26
Authors: Kooshapur, Hamed; Meyer, H.; Madl, T.; Sattler, M.
Citation: Neuhaus, Alexander; Kooshapur, Hamed; Wolf, Janina; Meyer, N. Helge; Madl, Tobias; Saidowsky, Jurgen; Hambruch, Eva; Lazam, Anissa; Jung, Martin; Sattler, Michael; Schliebs, Wolfgang; Erdmann, Ralf. "A Novel Pex14 Protein-interacting Site of Human Pex5 Is Critical for Matrix Protein Import into Peroxisomes." J. Biol. Chem. 289, 437-448 (2014).
Assembly members:
PEROXISOMAL_MEMBRANE_PROTEIN_PEX14, polymer, 69 residues, 7445.4517 Da.
PEROXISOMAL_TARGETING_SIGNAL_1_RECEPTOR, polymer, 15 residues, 1701.7162 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PEROXISOMAL_MEMBRANE_PROTEIN_PEX14: GAMATPGSENVLPREPLIAT
AVKFLQNSRVRQSPLATRRA
FLKKKGLTDEEIDMAFQQSG
TAADEPSSL
PEROXISOMAL_TARGETING_SIGNAL_1_RECEPTOR: ASEDELVAEFLQDQN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 415 |
| 15N chemical shifts | 124 |
| 1H chemical shifts | 847 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PEROXISOMAL MEMBRANE PROTEIN PEX14 | 1 |
| 2 | PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR | 2 |
Entities:
Entity 1, PEROXISOMAL MEMBRANE PROTEIN PEX14 69 residues - 7445.4517 Da.
| 1 | GLY | ALA | MET | ALA | THR | PRO | GLY | SER | GLU | ASN | ||||
| 2 | VAL | LEU | PRO | ARG | GLU | PRO | LEU | ILE | ALA | THR | ||||
| 3 | ALA | VAL | LYS | PHE | LEU | GLN | ASN | SER | ARG | VAL | ||||
| 4 | ARG | GLN | SER | PRO | LEU | ALA | THR | ARG | ARG | ALA | ||||
| 5 | PHE | LEU | LYS | LYS | LYS | GLY | LEU | THR | ASP | GLU | ||||
| 6 | GLU | ILE | ASP | MET | ALA | PHE | GLN | GLN | SER | GLY | ||||
| 7 | THR | ALA | ALA | ASP | GLU | PRO | SER | SER | LEU |
Entity 2, PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR 15 residues - 1701.7162 Da.
| 1 | ALA | SER | GLU | ASP | GLU | LEU | VAL | ALA | GLU | PHE | ||||
| 2 | LEU | GLN | ASP | GLN | ASN |
Samples:
sample_1: PEROXISOMAL MEMBRANE PROTEIN PEX14, [U-13C; U-15N], mM; PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR, [U-13C; U-15N], mM
sample_conditions_1: ionic strength: 0.0 mM; pH: 6.500; pressure: 1 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Experiment_1 | sample_1 | solution | sample_conditions_1 |
Software:
ARIA1.2_CNS vany, JP Linge, SI O Donoghue and M Nilges -
AutoDep v4.3 -
NMR spectrometers:
- Bruker Avance 0 MHz
Related Database Links:
| UNP | PEX14_HUMAN PEX5_HUMAN |
| PDB | |
| DBJ | BAA36837 BAE00503 BAE88740 BAG35878 BAG51028 |
| EMBL | CAG29317 CAG46880 |
| GB | AAC39843 AAH06327 AAX36586 ABM83701 ABM86831 |
| REF | NP_001271931 NP_004556 XP_001118790 XP_001162201 XP_002811556 XP_004052675 |
| SP | O75381 |
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