BMRB Entry 19449
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19449
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Title: NMR structure of a BolA-like hypothetical protein RP812 from RICKETTSIA PROWAZEKII, SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE (SSGCID)
Deposition date: 2013-08-22 Original release date: 2013-10-08
Authors: Chen, Yu; BARNWAL, RAVI; Yang, Fan; Varani, Gabriele
Citation: Chen, Yu; BARNWAL, Ravi; Yang, Fan; Varani, Gabriele. "NMR structure of a BolA-like hypothetical protein RP812 from RICKETTSIA PROWAZEKII, SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE (SSGCID)" To be Published ., .-..
Assembly members:
entity, polymer, 75 residues, 8541.002 Da.
Natural source: Common Name: a-proteobacteria Taxonomy ID: 782 Superkingdom: Bacteria Kingdom: Proteobacteria Genus/species: Rickettsia prowazekii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MAISAEELEKILKKSFPSSV
IKITDLVGDQDHYALEISDA
QFNGLSLINQHKLVKNALSE
ILNKKLHSISIKTIS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 320 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 509 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BolA-like hypothetical protein RP812 | 1 |
Entities:
Entity 1, BolA-like hypothetical protein RP812 75 residues - 8541.002 Da.
| 1 | MET | ALA | ILE | SER | ALA | GLU | GLU | LEU | GLU | LYS | ||||
| 2 | ILE | LEU | LYS | LYS | SER | PHE | PRO | SER | SER | VAL | ||||
| 3 | ILE | LYS | ILE | THR | ASP | LEU | VAL | GLY | ASP | GLN | ||||
| 4 | ASP | HIS | TYR | ALA | LEU | GLU | ILE | SER | ASP | ALA | ||||
| 5 | GLN | PHE | ASN | GLY | LEU | SER | LEU | ILE | ASN | GLN | ||||
| 6 | HIS | LYS | LEU | VAL | LYS | ASN | ALA | LEU | SER | GLU | ||||
| 7 | ILE | LEU | ASN | LYS | LYS | LEU | HIS | SER | ILE | SER | ||||
| 8 | ILE | LYS | THR | ILE | SER |
Samples:
sample_1: BolA-like hypothetical protein RP812, [U-98% 13C; U-98% 15N], 1.5 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection, processing
CcpNMR v2.3, CCPN - chemical shift assignment, peak picking
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA15238 |
| GB | ADE30378 AFE49607 AFE50451 AFE51295 AFE52133 |
| REF | NP_221162 WP_004596876 |
| SP | Q9ZCE4 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts