BMRB Entry 19453
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19453
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR structure of the protein ZP_02042476.1 from Ruminococcus gnavus
Deposition date: 2013-08-27 Original release date: 2013-11-11
Authors: Martin, Bryan; Serrano, Pedro; Geralt, Michael; Dutta, Samit; Wuthrich, Kurt
Citation: Martin, Bryan; Wuthrich, Kurt; Serrano, Pedro; Geralt, Michael; Dutta, Samit. "NMR structure of the protein ZP_02042476.1 from Ruminococcus gnavus." Not known ., .-..
Assembly members:
entity, polymer, 100 residues, 11710.976 Da.
Natural source: Common Name: firmicutes Taxonomy ID: 33038 Superkingdom: Bacteria Kingdom: Firmicutes Genus/species: Ruminococcus gnavus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: PILPKAENVDSICIDFTNSI
QKIYDDSESIQKILSEIATG
KRTEKQSIQDYPSAEEYGTI
NIENNGGMTTMFYYEENGKY
YIECPYKGIYEIENNFEDMI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 325 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 688 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SP17945A | 1 |
Entities:
Entity 1, SP17945A 100 residues - 11710.976 Da.
| 1 | PRO | ILE | LEU | PRO | LYS | ALA | GLU | ASN | VAL | ASP | |
| 2 | SER | ILE | CYS | ILE | ASP | PHE | THR | ASN | SER | ILE | |
| 3 | GLN | LYS | ILE | TYR | ASP | ASP | SER | GLU | SER | ILE | |
| 4 | GLN | LYS | ILE | LEU | SER | GLU | ILE | ALA | THR | GLY | |
| 5 | LYS | ARG | THR | GLU | LYS | GLN | SER | ILE | GLN | ASP | |
| 6 | TYR | PRO | SER | ALA | GLU | GLU | TYR | GLY | THR | ILE | |
| 7 | ASN | ILE | GLU | ASN | ASN | GLY | GLY | MET | THR | THR | |
| 8 | MET | PHE | TYR | TYR | GLU | GLU | ASN | GLY | LYS | TYR | |
| 9 | TYR | ILE | GLU | CYS | PRO | TYR | LYS | GLY | ILE | TYR | |
| 10 | GLU | ILE | GLU | ASN | ASN | PHE | GLU | ASP | MET | ILE |
Samples:
sample_1: entity, [U-98% 13C; U-98% 15N], 1.2 mM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 5 mM; D2O 5%; H2O 95%
sample_conditions_1: ionic strength: 0.220 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| APSY 4D-HACANH | sample_1 | isotropic | sample_conditions_1 |
| APSY 5D-CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| APSY 5D-HACACONH | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, G ntert, P. - structure solution
TOPSPIN, Bruker Biospin - collection, data analysis, processing
UNIO v2010, Herrmann ann Wuthrich - chemical shift assignment, peak picking, structure solution
CARA, Keller and Wuthrich - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| EMBL | CCZ67861 CUO51325 |
| GB | EDN76176 EGN49208 ETD16178 |
| REF | WP_004844251 WP_024854441 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts