BMRB Entry 19774
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19774
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone chemical shifts for the tandem UIMs of wild-type RAP80. PubMed: 24627472
Deposition date: 2014-02-06 Original release date: 2014-02-06
Authors: Anamika, Anamika; Markin, Craig; Rout, Manoj; Spyracopoulos, Leo
Citation: Anamika, Anamika; Markin, Craig; Rout, Manoj; Spyracopoulos, Leo. "Molecular Basis for Impaired DNA Damage Response Function Associated with the RAP80 E81 Defect." J. Biol. Chem. ., .-. (2014).
Assembly members:
entity, polymer, 63 residues, 7018.751 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GPLGSRKIAQMTEEEQFALA
LKMSEQEAREVNSQEEEEEE
LLRKAIAESLNSCRPSDASA
TRS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 152 |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 104 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 63 residues - 7018.751 Da.
| 1 | GLY | PRO | LEU | GLY | SER | ARG | LYS | ILE | ALA | GLN | ||||
| 2 | MET | THR | GLU | GLU | GLU | GLN | PHE | ALA | LEU | ALA | ||||
| 3 | LEU | LYS | MET | SER | GLU | GLN | GLU | ALA | ARG | GLU | ||||
| 4 | VAL | ASN | SER | GLN | GLU | GLU | GLU | GLU | GLU | GLU | ||||
| 5 | LEU | LEU | ARG | LYS | ALA | ILE | ALA | GLU | SER | LEU | ||||
| 6 | ASN | SER | CYS | ARG | PRO | SER | ASP | ALA | SER | ALA | ||||
| 7 | THR | ARG | SER |
Samples:
sample_1: RAP80-tUIM, [U-100% 13C; U-100% 15N], 0.33 mM; TRIS 50 mM; sodium chloride 150 mM; DTT 2 mM; H2O 90%; D2O 10%
sample_2: RAP80-tUIM, [U-100% 13C; U-100% 15N], 1.2 mM; TRIS 50 mM; sodium chloride 150 mM; DTT 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
VNMRJ, Varian - collection
SPARKY, Goddard - chemical shift assignment
CARA, Keller and Wuthrich - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 19773 |
| PDB | |
| GB | AAG59851 KFO81465 |
| REF | XP_006220429 XP_007124893 XP_008013593 XP_008988510 XP_009566349 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts