BMRB Entry 19828

Name: HUMAN CCR2 MEMBRANE-PROXIMAL C-TERMINAL REGION (PRO-C) IN A MEMBRANE BOUND FORM
Published: 2014-10-06

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PDB ID: 2mlo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19828
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: HUMAN CCR2 MEMBRANE-PROXIMAL C-TERMINAL REGION (PRO-C) IN A MEMBRANE BOUND FORM

Deposition date: 2014-03-04 Original release date: 2014-10-06

Authors: Esaki, Kaori

Citation: Esaki, K.. "Structural Insight Into the Interaction between the Membrane-Proximal C-Terminal Region of Chemokine Receptor Ccr2 and the Cytosolic Regulator Frount" To be Published ., .-..

Assembly members:
CHEMOKINE_(C-C_MOTIF)_RECEPTOR_2, polymer, 16 residues, 2133.564 Da.

Natural source: Common Name: HUMAN Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: HOMO SAPIENS

Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI

Entity Sequences (FASTA):
CHEMOKINE_(C-C_MOTIF)_RECEPTOR_2: EKFRRYLSVFFRKHIT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	71
15N chemical shifts	14
1H chemical shifts	124

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CHEMOKINE (C-C MOTIF) RECEPTOR 2	1

Entities:

Entity 1, CHEMOKINE (C-C MOTIF) RECEPTOR 2 16 residues - 2133.564 Da.

1

GLU

LYS

PHE

ARG

TYR

LEU

SER

VAL

PHE

2

PHE

ARG

LYS

HIS

ILE

THR

Samples:

CCR2_Pro-C_13C15N1: CCR2_Pro-C, [U-100% 13C; U-100% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; DPC, [U-99% 2H], 10 mM; D2O, [U-100% 2H], 100%

CCR2_Pro-C_13C15N2: CCR2_Pro-C, [U-100% 13C; U-100% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; DPC, [U-99% 2H], 10 mM; D2O, [U-100% 2H], 5%; H2O 95%

CCR2_Pro-C_15N: CCR2_Pro-C, [U-100% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; DPC, [U-99% 2H], 10 mM; D2O, [U-100% 2H], 5%; H2O 95%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	CCR2_Pro-C_15N	isotropic	sample_conditions_1
3D HNHA	CCR2_Pro-C_15N	isotropic	sample_conditions_1
3D 1H- 13C NOESY	CCR2_Pro-C_13C15N1	isotropic	sample_conditions_1
3D CBCA(CO)NH	CCR2_Pro-C_13C15N2	isotropic	sample_conditions_1
3D HNCACB	CCR2_Pro-C_13C15N2	isotropic	sample_conditions_1
3D HBHA(CO)NH	CCR2_Pro-C_13C15N2	isotropic	sample_conditions_1
3D HCCH- TOCSY	CCR2_Pro-C_13C15N1	isotropic	sample_conditions_1
2D 1H-15N HSQC	CCR2_Pro-C_15N	isotropic	sample_conditions_1
2D 1H-13C HSQC	CCR2_Pro-C_13C15N1	isotropic	sample_conditions_1

Software:

CYANA, GUNTERT, MUMENTHALER - structure solution

NMR spectrometers:

BRUKER AVANCE III 600 MHz
Bruker Avance 600 MHz

BMRB	19829
PDB	2MLO 2MLQ
DBJ	BAA06253 BAD08648 BAD08655 BAD12134 BAF85374
EMBL	CAA64835
GB	AAA19120 AAB57792 AAC51636 AAH95540 ACN78985
REF	NP_001001619 NP_001116868 XP_002832824 XP_003483213 XP_003926313

Biological Magnetic Resonance Data Bank