BMRB Entry 19829
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19829
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Title: HUMAN CCR2 MEMBRANE-PROXIMAL C-TERMINAL REGION (PRO-C) IN A FROUNT BOUND FORM
Deposition date: 2014-03-04 Original release date: 2014-10-06
Authors: Esaki, Kaori
Citation: Esaki, K.. "Structural Insight Into the Interaction between the Membrane-Proximal C- Terminal Region of Chemokine Receptor Ccr2 and the Cytosolic Regulator Frount" To be Published ., .-..
Assembly members:
CHEMOKINE_(C-C_MOTIF)_RECEPTOR_2, polymer, 16 residues, 2133.564 Da.
Natural source: Common Name: HUMAN Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: HOMO SAPIENS
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
CHEMOKINE_(C-C_MOTIF)_RECEPTOR_2: EKFRRYLSVFFRKHIT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 39 |
| 15N chemical shifts | 14 |
| 1H chemical shifts | 110 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CHEMOKINE (C-C MOTIF) RECEPTOR 2 | 1 |
Entities:
Entity 1, CHEMOKINE (C-C MOTIF) RECEPTOR 2 16 residues - 2133.564 Da.
| 1 | GLU | LYS | PHE | ARG | ARG | TYR | LEU | SER | VAL | PHE | ||||
| 2 | PHE | ARG | LYS | HIS | ILE | THR |
Samples:
CCR2_Pro-C_15N_FROUNT: CCR2_Pro-C, [U-100% 15N], 800 uM; sodium phosphate 20 mM; sodium chloride 50 mM; FROUNT 8 uM; D2O, [U-100% 2H], 5%; H2O 95%
CCR2_Pro-C_13C15N_FROUNT: CCR2_Pro-C, [U-100% 13C; U-100% 15N], 800 uM; sodium phosphate 20 mM; sodium chloride 50 mM; FROUNT 8 uM; D2O, [U-100% 2H], 100%
CCR2_Pro-C_13C15N: CCR2_Pro-C, [U-100% 13C; U-100% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; D2O, [U-100% 2H], 5%; H2O 95%
CCR2_Pro-C_13C15N2: CCR2_Pro-C, [U-100% 13C; U-100% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; D2O, [U-100% 2H], 100%
CCR2_Pro-C_15N2: CCR2_Pro-C, [U-100% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; D2O, [U-100% 2H], 5%; H2O 95%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 268.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | CCR2_Pro-C_15N_FROUNT | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | CCR2_Pro-C_13C15N_FROUNT | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | CCR2_Pro-C_13C15N | isotropic | sample_conditions_1 |
| 3D HNCACB | CCR2_Pro-C_13C15N | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | CCR2_Pro-C_13C15N | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | CCR2_Pro-C_13C15N2 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | CCR2_Pro-C_15N2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | CCR2_Pro-C_13C15N2 | isotropic | sample_conditions_1 |
Software:
CYANA, GUNTERT, MUMENTHALER - refinement
NMR spectrometers:
- BRUKER AVANCE III 600 MHz
Related Database Links:
| BMRB | 19828 |
| PDB | |
| DBJ | BAA06253 BAD08648 BAD08655 BAD12134 BAF85374 |
| EMBL | CAA64835 |
| GB | AAA19120 AAB57792 AAC51636 AAH95540 ACN78985 |
| REF | NP_001001619 NP_001116868 XP_002832824 XP_003483213 XP_003926313 |
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