BMRB Entry 19959
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19959
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Title: Structural basis for binding of Pan3 to Pan2 and its function in mRNA recruitment and deadenylation
Deposition date: 2014-05-08 Original release date: 2014-05-20
Authors: Wolf, J.; Valkov, E.; Allen, Mark; Meineke, B.; Gordiyenko, Y.; Mclaughlin, S.; Olsen, T.; Robinson, C.; Bycroft, M.; Stewart, M.; Passmore, L.; Olsen, S.
Citation: Wolf, J.; Valkov, E.; Allen, Mark; Meineke, B.; Gordiyenko, Y.; Mclaughlin, S.; Olsen, T.; Robinson, C.; Bycroft, M.; Stewart, M.; Passmore, L.. "Structural basis for binding of Pan3 to Pan2 and its function in mRNA recruitment and deadenylation" To Be Published ., .-..
Assembly members:
PAB-DEPENDENT_POLY(A)-SPECIFIC_RIBONUCLEASE_SUBUN, polymer, 41 residues, 4715.2286 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI B41
Entity Sequences (FASTA):
PAB-DEPENDENT_POLY(A)-SPECIFIC_RIBONUCLEASE_SUBUN: MDKINPDWAKDIPCRNITIY
GYCKKEKEGCPFKHSDNTTA
T
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 285 |
| 13C chemical shifts | 121 |
| 15N chemical shifts | 36 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUN | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUN 41 residues - 4715.2286 Da.
| 1 | MET | ASP | LYS | ILE | ASN | PRO | ASP | TRP | ALA | LYS | ||||
| 2 | ASP | ILE | PRO | CYS | ARG | ASN | ILE | THR | ILE | TYR | ||||
| 3 | GLY | TYR | CYS | LYS | LYS | GLU | LYS | GLU | GLY | CYS | ||||
| 4 | PRO | PHE | LYS | HIS | SER | ASP | ASN | THR | THR | ALA | ||||
| 5 | THR |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUN, [U-13C; U-15N], 1.5 mM; Tris 20 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 100.000 mM; pH: 7.000; pressure: 1 atm; temperature: 293.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| na | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - collection
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Bruker Avance . MHz
Related Database Links:
| GB | EGA85959.1 AHY76216 AJP40010 AJS30208 AJS30509 AJS30808 |
| UNP | PAN3_YEAST |
| PDB | |
| DBJ | GAA24697 |
| EMBL | CAA81860 CAY81058 |
| REF | NP_012900 |
| SP | P36102 |
| TPG | DAA09129 |
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