BMRB Entry 25096
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25096
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Title: Solution Structure of MciZ from Bacillus subtilis
Deposition date: 2014-07-16 Original release date: 2015-03-10
Authors: Castellen, Patricia; Sforca, Mauricio; Zeri, Ana; Gueiros-Filho, Frederico
Citation: Bisson-Filho, Alexandre; Discola, Karen; Castellen, Patricia; Blasios, Valdir; Martins, Alexandre; Sforca, Mauricio; Garcia, Wanius; Zeri, Ana; Erickson, Harold; Dessen, Andrea; Gueiros-Filho, Frederico. "Filament capping regulates the bacterial FtsZ cytoskeleton" Not known ., .-..
Assembly members:
entity, polymer, 40 residues, 4785.832 Da.
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: MKVHRMPKGVVLVGKAWEIR
AKLKEYGRTFQYVKDWISKP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 263 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MciZ from Bacillus subtilis | 1 |
Entities:
Entity 1, MciZ from Bacillus subtilis 40 residues - 4785.832 Da.
| 1 | MET | LYS | VAL | HIS | ARG | MET | PRO | LYS | GLY | VAL | |
| 2 | VAL | LEU | VAL | GLY | LYS | ALA | TRP | GLU | ILE | ARG | |
| 3 | ALA | LYS | LEU | LYS | GLU | TYR | GLY | ARG | THR | PHE | |
| 4 | GLN | TYR | VAL | LYS | ASP | TRP | ILE | SER | LYS | PRO |
Samples:
sample_1: entity 0.2 mM; sodium phosphate 50 mM; potassium chloride 50 mM
sample_2: entity 0.2 mM; sodium phosphate 50 mM; potassium chloride 50 mM
sample_conditions_1: temperature: 298 K; pH: 4.0; pressure: 1 atm; ionic strength: 0.05 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment, chemical shift calculation, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, chemical shift assignment, processing
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution, refinement
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Agilent INOVA 600 MHz