BMRB Entry 25149
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25149
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Title: Isolated Ring domain PubMed: 25306923
Deposition date: 2014-08-13 Original release date: 2014-10-27
Authors: Brown, Nicholas; Watson, Edmond; Weissman, Florian; Royappa, Grace; Schulman, Brenda; Jarvis, Marc; Vanderlinden, Ryan; Frye, Jeremiah; Qiao, Renping; Petzold, Georg; Peters, Jan-Michael; Stark, Holger
Citation: Brown, Nicholas; Watson, Edmond; Weissman, Florian; Royappa, Grace; Jarvis, Marc; Vanderlinden, Ryan; Frye, Jeremiah; Qiao, Renping; Petzold, Georg; Peters, Jan-Michael; Stark, Holger; Schulman, Brenda. "Mechanism of Polyubiquitination by Human Anaphase-Promoting Complex: RING Repurposing for Ubiquitin Chain Assembly" Mol. Cell ., .-. (2014).
Assembly members:
entity_1, polymer, 70 residues, 8191.060 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: GSVANDENCGICRMAFNGCC
PDCKVPGDDCPLVWGQCSHC
FHMHCILKWLHAQQVQQHCP
MCRQEWKFKE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 469 |
| 13C chemical shifts | 292 |
| 15N chemical shifts | 76 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | Zinc Ion 1 | 2 |
| 3 | Zinc Ion 2 | 2 |
| 4 | Zinc Ion 3 | 2 |
Entities:
Entity 1, entity_1 70 residues - 8191.060 Da.
| 1 | GLY | SER | VAL | ALA | ASN | ASP | GLU | ASN | CYS | GLY | |
| 2 | ILE | CYS | ARG | MET | ALA | PHE | ASN | GLY | CYS | CYS | |
| 3 | PRO | ASP | CYS | LYS | VAL | PRO | GLY | ASP | ASP | CYS | |
| 4 | PRO | LEU | VAL | TRP | GLY | GLN | CYS | SER | HIS | CYS | |
| 5 | PHE | HIS | MET | HIS | CYS | ILE | LEU | LYS | TRP | LEU | |
| 6 | HIS | ALA | GLN | GLN | VAL | GLN | GLN | HIS | CYS | PRO | |
| 7 | MET | CYS | ARG | GLN | GLU | TRP | LYS | PHE | LYS | GLU |
Entity 2, Zinc Ion 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: entity_1, [U-100% 13C; U-100% 15N], 0.5 mM; H2O 90%; D2O 10%
sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm; ionic strength: 100 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
cyana v2.1, Guntert, Mumenthaler and Wuthrich - structure solution, data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB22663 BAB22890 BAB22937 BAC32348 BAE22944 |
| EMBL | CAH92008 |
| GB | AAF65816 AAH23039 AAH66308 AAH95454 AAI02427 |
| REF | NP_001002245 NP_001002246 NP_001002247 NP_001002248 NP_001002249 |
| SP | Q3ZCF6 Q5R8A2 Q9CPX9 Q9NYG5 |
| TPG | DAA14168 DAA18262 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts