BMRB Entry 25289
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25289
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Title: Atomic-resolution 3D structure of amyloid-beta fibrils: the Osaka mutation PubMed: 25395337
Deposition date: 2014-10-17 Original release date: 2014-11-24
Authors: Schuetz, Anne; Vagt, Toni; Huber, Matthias; Ovchinnikova, Oxana; Cadalbert, Riccardo; Wall, Joseph; Guentert, Peter; Bockmann, Anja; Glockshuber, Rudi; Meier, Beat
Citation: Schuetz, Anne; Vagt, Toni; Huber, Matthias; Ovchinnikova, Oxana; Cadalbert, Riccardo; Wall, Joseph; Guentert, Peter; Bockmann, Anja; Glockshuber, Rudi; Meier, Beat. "Atomic-Resolution Three-Dimensional Structure of Amyloid beta Fibrils Bearing the Osaka Mutation" Angew. Chem. Int. Ed. Engl. 54, 331-335 (2015).
Assembly members:
amyloid_beta, polymer, 39 residues, 4206.777 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
amyloid_beta: DAEFRHDSGYEVHHQKLVFF
ADVGSNKGAIIGLMVGGVV
| Data type | Count |
| 13C chemical shifts | 477 |
| 15N chemical shifts | 91 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 1 |
| 3 | entity_3 | 1 |
| 4 | entity_4 | 1 |
| 5 | entity_5 | 1 |
| 6 | entity_6 | 1 |
| 7 | entity_7 | 1 |
| 8 | entity_8 | 1 |
| 9 | entity_9 | 1 |
| 10 | entity_10 | 1 |
Entities:
Entity 1, entity_1 39 residues - 4206.777 Da.
| 1 | ASP | ALA | GLU | PHE | ARG | HIS | ASP | SER | GLY | TYR | ||||
| 2 | GLU | VAL | HIS | HIS | GLN | LYS | LEU | VAL | PHE | PHE | ||||
| 3 | ALA | ASP | VAL | GLY | SER | ASN | LYS | GLY | ALA | ILE | ||||
| 4 | ILE | GLY | LEU | MET | VAL | GLY | GLY | VAL | VAL |
Samples:
uniform_13C15N: amyloid beta, [U-100% 13C; U-100% 15N], 15 mg
uniform_13C: amyloid beta, [U-100% 13C], 15 mg
mixed_13C-15N: amyloid beta, [U-100% 13C; U-100% 15N], 15 mg
2-13C-glucose: amyloid beta, [U-100% 2-13C-glucose; U-100% 15N], 15 mg
diluted: amyloid beta, [U-100% 13C; U-100% 15N]/natural abundance, 15 mg
sample_conditions_1: ionic strength: 0 M; pH: 7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NCA | uniform_13C15N | solid | sample_conditions_1 |
| NCO | uniform_13C15N | solid | sample_conditions_1 |
| DARR 15 ms | uniform_13C | solid | sample_conditions_1 |
| PAIN 6 ms | uniform_13C15N | solid | sample_conditions_1 |
| PAIN 6 ms | mixed_13C-15N | solid | sample_conditions_1 |
| DARR 400 ms | uniform_13C | solid | sample_conditions_1 |
| DARR 400 ms | diluted | solid | sample_conditions_1 |
| PAR 8 ms | uniform_13C | solid | sample_conditions_1 |
| PAR 8 ms | diluted | solid | sample_conditions_1 |
| CHHC 500 us | uniform_13C | solid | sample_conditions_1 |
| CHHC 500 us | diluted | solid | sample_conditions_1 |
| PDSD 4 s | 2-13C-glucose | solid | sample_conditions_1 |
Software:
CYANA v3.9.6, Guntert, Mumenthaler and Wuthrich - structure solution
CcpNMR v2.2.2, CCPN - chemical shift assignment, data analysis, peak picking
TALOS+ v2.1, Cornilescu, Delaglio and Bax - dihedral angle prediction
NMR spectrometers:
- Bruker Avance 850 MHz
Related Database Links:
| BMRB | 11435 15775 17159 17186 17764 17793 17794 17795 17796 18052 18127 18128 18129 18131 19009 19309 19393 25218 25429 26508 26516 |
| PDB | |
| DBJ | BAA22264 BAA84580 BAD51938 BAE01907 BAG10647 |
| EMBL | CAA30050 CAA31830 CAA39589 CAA39590 CAA39591 |
| GB | AAA35540 AAA36829 AAA51722 AAA51726 AAA51727 |
| PIR | A60045 D60045 E60045 G60045 PQ0438 |
| PRF | 1303338A 1403400A 1405204A 1507304A 1507304B |
| REF | NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_001127014 |
| SP | P05067 P53601 P79307 Q28053 Q28280 |
| TPG | DAA33655 |