BMRB Entry 25402

Name: Solution structure of the full length sorting nexin 3
Published: 2015-08-13

Click here to enlarge.

PDB ID: 2mxc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25402
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Solution structure of the full length sorting nexin 3 PubMed: 25893673

Deposition date: 2014-12-20 Original release date: 2015-08-13

Authors: Lenoir, Marc; Rajesh, Sandya; Gruenberg, Jean; Overduin, Michael; Kaur, Jaswant

Citation: Overduin, Michael; Rajesh, Sandya; Gruenberg, Jean; Lenoir, Marc. "Secondary structure and 1H, 13C, 15N resonance assignments of the endosomal sorting protein sorting nexin 3" Biomol. NMR Assign. 9, 355-358 (2015).

Assembly members:
SNX3FL, polymer, 172 residues, 19924.6031 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
SNX3FL: MAHHHHHHVGTAETVADTRR LITKPQNLNDAYGPPSNFLE IDVSNPQTVGVGRGRFTTYE IRVKTNLPIFKLKESTVRRR YSDFEWLRSELERESKVVVP PLPGKAFLRQLPFRGDDGIF DDNFIEERKQGLEQFINKVA GHPLAQNERCLHMFLQDEII DKSYTPSKIRHA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	729
15N chemical shifts	165
1H chemical shifts	1177

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SNX3FL	1

Entities:

Entity 1, SNX3FL 172 residues - 19924.6031 Da.

1

MET

ALA

HIS

VAL

GLY

2

THR

ALA

GLU

THR

VAL

ALA

ASP

THR

ARG

3

LEU

ILE

THR

LYS

PRO

GLN

ASN

LEU

ASN

ASP

4

ALA

TYR

GLY

PRO

SER

ASN

PHE

LEU

GLU

5

ILE

ASP

VAL

SER

ASN

PRO

GLN

THR

VAL

GLY

6

VAL

GLY

ARG

GLY

ARG

PHE

THR

TYR

GLU

7

ILE

ARG

VAL

LYS

THR

ASN

LEU

PRO

ILE

PHE

8

LYS

LEU

LYS

GLU

SER

THR

VAL

ARG

9

TYR

SER

ASP

PHE

GLU

TRP

LEU

ARG

SER

GLU

10

LEU

GLU

ARG

GLU

SER

LYS

VAL

PRO

11

PRO

LEU

PRO

GLY

LYS

ALA

PHE

LEU

ARG

GLN

12

LEU

PRO

PHE

ARG

GLY

ASP

GLY

ILE

PHE

13

ASP

ASN

PHE

ILE

GLU

ARG

LYS

GLN

14

GLY

LEU

GLU

GLN

PHE

ILE

ASN

LYS

VAL

ALA

15

GLY

HIS

PRO

LEU

ALA

GLN

ASN

GLU

ARG

CYS

16

LEU

HIS

MET

PHE

LEU

GLN

ASP

GLU

ILE

17

ASP

LYS

SER

TYR

THR

PRO

SER

LYS

ILE

ARG

18

HIS

ALA

Samples:

Assignments: SNX3FL, [U-100% 13C; U-100% 15N], 0.5 mM; NaCl 100 mM; Sodium phosphate 20 mM

CondSet1: ionic strength: 0.100 M; pH: 6.5; pressure: 1.000 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	Assignments	isotropic	CondSet1
3D HNCA	Assignments	isotropic	CondSet1
3D HN(CO)CA	Assignments	isotropic	CondSet1
3D HNCO	Assignments	isotropic	CondSet1
3D CBCA(CO)NH	Assignments	isotropic	CondSet1
3D HNCACB	Assignments	isotropic	CondSet1
3D HNCO	Assignments	isotropic	CondSet1
3D HN(CA)CO	Assignments	isotropic	CondSet1
3D HCCH-TOCSY	Assignments	isotropic	CondSet1
3D 1H-13C NOESY	Assignments	isotropic	CondSet1
3D 1H-15N NOESY	Assignments	isotropic	CondSet1
2D 1H-13C HSQC	Assignments	isotropic	CondSet1

Software:

CcpNmr_Analysis v2.2, CCPN, Linge, O'Donoghue and Nilges - chemical shift calculation, data analysis, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian UnityInova 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts