BMRB Entry 25564

Assembly:

Entities:

Entity 1, entity 106 residues - 11337.317 Da.

The recombinant protein contains six extraneous amino acids at the N-terminus, residues 808-905 of human fibronectin, and two extraneous amino acids at the C-terminus (gshmgtTTAP...PRSDgt).

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N], 0.6 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; sodium chloride 140 mM; potassium chloride 2.7 mM; H2O 90%; D2O 10%

sample_2: entity, [U-99% 15N], 0.6 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; sodium chloride 140 mM; potassium chloride 2.7 mM; H2O 90%; D2O 10%

sample_3: entity 0.6 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; sodium chloride 140 mM; potassium chloride 2.7 mM; H2O 90%; D2O 10%

sample_4: entity 0.6 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; sodium chloride 140 mM; potassium chloride 2.7 mM; D2O 100%

sample_5: entity, [U-99% 13C; U-99% 15N], 0.9 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; sodium chloride 140 mM; potassium chloride 2.7 mM; entity 0.9 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.17 M; pH: 7.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

Software:

VNMRJ, Agilent - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr, CCPN - chemical shift assignment, data analysis, peak picking

ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

TALOS, Cornilescu, Delaglio and Bax - generation of torsion angle restraints

NMR spectrometers:

• Varian Varian NMR System 600 MHz
• Varian Varian NMR System 800 MHz