BMRB Entry 25595
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25595
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Title: NMR Structure of TDP-43 prion-like hydrophobic helix in DPC
Deposition date: 2015-05-06 Original release date: 2015-11-19
Authors: Lim, Liang Zhong; Song, Jianxing
Citation: Lim, Liang Zhong; Song, Jianxing. "ALS-causing mutations significantly perturb the self-assembly and interaction with nucleic acid of the intrinsically-disordered prion-like domain of TDP-43" Plos Biology ., .-..
Assembly members:
entity, polymer, 43 residues, 4336.979 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MGGGMNFGAFSINPAMMAAA
QAALQSSWGMMGMLASQQNQ
SGP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 84 |
| 15N chemical shifts | 40 |
| 1H chemical shifts | 188 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 43 residues - 4336.979 Da.
| 1 | MET | GLY | GLY | GLY | MET | ASN | PHE | GLY | ALA | PHE | ||||
| 2 | SER | ILE | ASN | PRO | ALA | MET | MET | ALA | ALA | ALA | ||||
| 3 | GLN | ALA | ALA | LEU | GLN | SER | SER | TRP | GLY | MET | ||||
| 4 | MET | GLY | MET | LEU | ALA | SER | GLN | GLN | ASN | GLN | ||||
| 5 | SER | GLY | PRO |
Samples:
sample_1: entity, [U-100% 15N], 300 uM; DPC 60 mM
sample_conditions_1: ionic strength: 1 mM; pH: 4; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| DBJ | BAC27753 BAD96474 BAE21557 BAE26823 BAE32189 |
| EMBL | CAB43367 CAG31358 CAG38565 CAH92854 CAJ81570 |
| GB | AAA70033 AAH12873 AAH25544 AAH27772 AAH31126 |
| REF | NP_001026049 NP_001039950 NP_001127597 NP_001231687 NP_001231688 |
| SP | Q13148 Q28F51 Q5R5W2 Q5ZLN5 Q921F2 |
| TPG | DAA21291 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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