BMRB Entry 26674
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26674
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Title: Structural basis for the inhibition of voltage-gated sodium channels with conotoxin-muOxi-GVIIJ PubMed: 26817840
Deposition date: 2015-09-30 Original release date: 2016-02-01
Authors: Green, Brad; Chhabra, Sandeep; Norton, Raymond
Citation: Green, Brad; Gajewiak, Joanna; Chhabra, Sandeep; Skalicky, Jack; Zhang, Min-Min; Bulaj, Grzegorz; Olivera, Baldomero; Yoshikami, Doju; Norton, Raymond. "Structural basis for the inhibition of voltage-gated sodium channels with conotoxin-muOxi-GVIIJ" J. Biol. Chem. 291, 7205-7220 (2016).
Assembly members:
GVIIJ_C24S, polymer, 35 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
GVIIJ_C24S: GWCGDXGATCGKLRLYCCSG
FCDSYTKTCKDKSSA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 63 |
| 15N chemical shifts | 31 |
| 1H chemical shifts | 202 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GVIIJ[C24S] | 1 |
Entities:
Entity 1, GVIIJ[C24S] 35 residues - Formula weight is not available
| 1 | GLY | TRP | CYS | GLY | ASP | HYP | GLY | ALA | THR | CYS | ||||
| 2 | GLY | LYS | LEU | ARG | LEU | TYR | CYS | CYS | SER | GLY | ||||
| 3 | PHE | CYS | ASP | SER | TYR | THR | LYS | THR | CYS | LYS | ||||
| 4 | ASP | LYS | SER | SER | ALA |
Samples:
sample_1: GVIIJ_C24S 1.4 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 10 mM; pH: 3.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr v2.1.5, CCPN - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts