BMRB Entry 30509
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30509
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Title: HS02 - Intragenic antimicrobial peptides derived from the protein unconventional myosin 1h
Deposition date: 2018-08-30 Original release date: 2019-08-06
Authors: Santos, M.; Brand, G.; Oliveira, A.
Citation: Ramada, M.; Manickchand, J.; Correa, R.; Ribeiro, D.; Santos, M.; Vasconcelos, A.; Prates, M.; Murad, A.; Filho, J.; Leite, J.; Magalhaes, K.; Oliveira, A.; Bloch Jr., C.; Brand, G.. "A surrogate innate immune system for the human skin composed by intragenic antimicrobial peptides" . ., .-..
Assembly members:
entity_1, polymer, 16 residues, 1967.469 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: KWAVRIIRKFIKGFIS
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 120 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 16 residues - 1967.469 Da.
| 1 | LYS | TRP | ALA | VAL | ARG | ILE | ILE | ARG | LYS | PHE | ||||
| 2 | ILE | LYS | GLY | PHE | ILE | SER |
Samples:
sample_1: peptide 2 ± 0.2 uM; Dodecylphosphorylcholine-d38, D-98%, 50 ± 0.2 uM; PBS 10 ± 0.2 mM
sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v2.3.1, Brunger, Adams, Clore, Gros, Nilges and Read, Linge, O'Donoghue and Nilges - refinement, structure calculation
CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation
CcpNMR v2.4, CCPN - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance III HD 600 MHz