BMRB Entry 30672
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30672
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Title: SMARCB1 nucleosome-interacting C-terminal alpha helix
Deposition date: 2019-09-16 Original release date: 2019-11-22
Authors: Valencia, A.; Sun, Z.; Seo, H.; Mashtalir, N.; Dhe-Paganon, S.; Kadoch, C.
Citation: Valencia, A.; Sun, Z.; Seo, H.; Mashtalir, N.; Dhe-Paganon, S.; Kadoch, C.. "SMARCB1 nucleosome-interacting C-terminal alpha helix" . ., .-..
Assembly members:
entity_1, polymer, 41 residues, 4748.473 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: GPLGSMPLLETLTDAEMEKK
IRDQDRNTRRMRRLANTAPA
W
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 172 |
| 15N chemical shifts | 41 |
| 1H chemical shifts | 282 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 41 residues - 4748.473 Da.
| 1 | GLY | PRO | LEU | GLY | SER | MET | PRO | LEU | LEU | GLU | ||||
| 2 | THR | LEU | THR | ASP | ALA | GLU | MET | GLU | LYS | LYS | ||||
| 3 | ILE | ARG | ASP | GLN | ASP | ARG | ASN | THR | ARG | ARG | ||||
| 4 | MET | ARG | ARG | LEU | ALA | ASN | THR | ALA | PRO | ALA | ||||
| 5 | TRP |
Samples:
sample_1: SMARCB1CC, [U-100% 13C; U-100% 15N], 300 uM; NaCl 137 mM; potassium chloride 2.7 mM; Na2HPO4 10 mM; KH2PO4 1.8 mM
sample_2: SMARCB1CC 230 uM; NaCl 137 mM; potassium chloride 2.7 mM; Na2HPO4 10 mM; KH2PO4 1.8 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
hmsIST, Hyberts and Wagner - processing
CARA, Keller and Wuthrich - data analysis
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Agilent DD2 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts