BMRB Entry 30712
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30712
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Title: Solution NMR of Prochlorosin 1.1 produced by Prochlorococcus MIT 9313 PubMed: 22574919
Deposition date: 2020-01-09 Original release date: 2020-07-05
Authors: Bobeica, S.; van der Donk, W.; Tang, W.
Citation: Tang, W.; van der Donk, W.. "Structural characterization of four prochlorosins: a novel class of lantipeptides produced by planktonic marine cyanobacteria." Biochemistry 51, 4271-4279 (2012).
Assembly members:
entity_1, polymer, 16 residues, 1789.109 Da.
Natural source: Common Name: Prochlorococcus marinus Taxonomy ID: 74547 Superkingdom: Bacteria Kingdom: not available Genus/species: Prochlorococcus marinus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: FFCVQGXANRFXINVC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 16 residues - 1789.109 Da.
| 1 | PHE | PHE | CYS | VAL | GLN | GLY | DBB | ALA | ASN | ARG | ||||
| 2 | PHE | DBB | ILE | ASN | VAL | CYS |
Samples:
sample_1: Prochlorosin 1.1 2 ± 0.2 mM
sample_2: Prochlorosin 1.1 2 ± 0.2 mM
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 296 K
sample_conditions_2: pH: 6; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Sparky, Goddard - chemical shift assignment, peak picking
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation
NMR spectrometers:
- Varian INOVA 600 MHz